Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Open Vet J. 2024 Apr;14(4):1043-1050. doi: 10.5455/OVJ.2024.v14.i4.11. Epub 2024 Apr 30.
Dengue infection can trigger an immunological response that results in an inflammatory reaction, which acts as a defensive mechanism to protect the host. Dengue infection leads to an elevation in the release of pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6). These three cytokines have been shown to correlate with the development of thrombocytopenia and plasma leakage, which is related to the severity of the disease.
This study aims to investigate the effect of faloak ( R. Br) stem bark on TNF-α, IL-1β, and IL-6 levels in Wistar rats infected with dengue, specifically DENV-3.
A group of 27 male Wistar rats () aged 2-3 months and weighting 200-300 g were divided into three distinct groups: healthy, dengue, and treatment (dengue infection and extract) groups. The rats in both the dengue and treatment groups were administered an injection of DENV-3 with a titer of 105 pfu at a dosage of 0.8 cc via the intraperitoneal route. The propagation of DENV-3 was initiated using C6/36 cells, and it underwent four passages. The extract was administered orally via a nasogastric tube at a dosage of 1,500 mg/kg body weight once daily for 7 days. The healthy group underwent blood sampling on the first day, whereas the dengue and therapy groups underwent blood sampling on the fifth and eighth, respectively.
Compared with the healthy group, TNF-α levels in the dengue and treatment groups showed significant differences on day 5 post-infection. The analysis revealed a statistically significant difference between the dengue-treatment and dengue-healthy groups. The IL-1β levels in the dengue and healthy groups significantly differed on days 5 and 8 post-infection compared to the healthy group. The treatment group had less of a decrease in IL-6 levels on days 5 and 8 than the dengue group. However, no statistically significant differences were observed.
The stem bark of shows potential as an anti-inflammatory agent in dengue infections, particularly in its ability to decrease levels of TNF-α and IL-1β.
登革热感染会引发免疫反应，导致炎症反应，这是宿主保护自身的防御机制。登革热感染会导致促炎细胞因子（包括肿瘤坏死因子-α[TNF-α]、白细胞介素-1β[IL-1β]和白细胞介素-6[IL-6]）的释放增加。这三种细胞因子与血小板减少症和血浆渗漏的发展有关，而后者与疾病的严重程度有关。
本研究旨在探讨法罗克（R. Br）树皮对感染登革热（特别是 DENV-3）的 Wistar 大鼠 TNF-α、IL-1β 和 IL-6 水平的影响。
将 27 只雄性 Wistar 大鼠（ ）分为三组：健康组、登革热组和治疗组（登革热感染和提取物组）。登革热组和治疗组大鼠均通过腹腔注射 105 pfu 滴度的 DENV-3 病毒液 0.8 cc，DENV-3 通过 C6/36 细胞进行繁殖，并经过 4 次传代。提取物通过鼻胃管以 1500 mg/kg 体重的剂量每日 1 次给药，共 7 天。健康组大鼠在第 1 天采血，登革热组和治疗组大鼠分别在第 5 天和第 8 天采血。
与健康组相比，登革热组和治疗组在感染后第 5 天 TNF-α 水平有显著差异。 分析显示，登革热治疗组与登革热健康组之间存在统计学差异。与健康组相比，登革热组和健康组在感染后第 5 天和第 8 天的 IL-1β 水平有显著差异。与登革热组相比，治疗组在第 5 天和第 8 天的 IL-6 水平下降幅度较小，但无统计学差异。
树皮具有作为抗登革热感染的抗炎剂的潜力，特别是在降低 TNF-α 和 IL-1β 水平方面。
