Centro de Referencia en Levaduras y Tecnología Cervecera (CRELTEC), Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales (IPATEC), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional del Comahue, San Carlos de Bariloche, Río Negro, Argentina.
Programa de Investigación en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa Rica.
Vet Microbiol. 2020 Jun;245:108703. doi: 10.1016/j.vetmic.2020.108703. Epub 2020 Apr 29.
Brucellosis in rams is caused by Brucella ovis or Brucella melitensis and it is considered one of the most important infectious diseases of males in sheep-raising countries. Molecular characterization of Brucella spp. achieved by multi-locus variable number of tandem repeats analysis (MLVA) is a powerful tool to genotype Brucella spp. However, data regarding B. ovis genotyping is scarce. Thus, the aim of this study was to characterize the molecular diversity of B. ovis field-strains in Argentina. A total of 115 isolates of B. ovis from Argentina and Uruguay were genotyped using MLVA-16 and analyzed altogether with 14 publicly available B. ovis genotypes from Brazil. The Discriminatory Power (D) was 0.996 for MLVA-16 and 0.0998 for MLVA-8 and MLVA-11. Analysis of MLVA-16 revealed 100 different genotypes, all of them novel, including 90 unique ones. There was no correlation between geographical distribution and genotype and results showed a higher diversity within provinces than between provinces. Clustering analysis of the strains from Argentina, Uruguay and Brazil revealed that the 129 isolates were grouped into two clades. Whole Genome Sequencing analysis of the 19 B. ovis genomes available in public databases, and including some of the Argentinian strains used in this study, revealed clustering of the Argentinian isolates and closer relationship with B. ovis from New Zealand and Australia. This work adds new data to the poorly understood distribution map of genotypes regionally and worldwide for B. ovis and it constitutes the largest study of B. ovis molecular genotyping until now.
绵羊布鲁氏菌病由绵羊布鲁氏菌或绵羊布鲁氏菌引起，被认为是绵羊养殖国家雄性动物中最重要的传染病之一。通过多位点可变串联重复分析（MLVA）对布鲁氏菌属进行分子特征分析是对布鲁氏菌属进行基因分型的有力工具。然而，关于绵羊布鲁氏菌基因分型的数据很少。因此，本研究旨在对阿根廷绵羊布鲁氏菌野毒株的分子多样性进行特征分析。使用 MLVA-16 对来自阿根廷和乌拉圭的 115 株绵羊布鲁氏菌进行基因分型，并与来自巴西的 14 株公开的绵羊布鲁氏菌基因型进行了综合分析。MLVA-16 的鉴别力（D）为 0.996，MLVA-8 和 MLVA-11 的鉴别力为 0.0998。MLVA-16 分析显示有 100 种不同的基因型，均为新基因型，其中包括 90 种独特的基因型。地理分布与基因型之间没有相关性，结果显示省内的多样性高于省际间的多样性。对来自阿根廷、乌拉圭和巴西的菌株进行聚类分析表明，129 株分离株分为两个分支。对公共数据库中可用的 19 株绵羊布鲁氏菌全基因组序列进行分析，包括本研究中使用的一些阿根廷菌株，结果显示阿根廷分离株聚类，并与新西兰和澳大利亚的绵羊布鲁氏菌关系更密切。这项工作增加了关于绵羊布鲁氏菌基因型在区域和全球范围内分布的了解，是迄今为止对绵羊布鲁氏菌分子基因分型的最大研究。
