VetPath Study Group, CEESA, 168 Av. de Tervueren, B-1150 Brussels, Belgium; Vétoquinol SA, Lure, France.
VetPath Study Group, CEESA, 168 Av. de Tervueren, B-1150 Brussels, Belgium; Virbac, Carros, France.
Vet Microbiol. 2020 Jun;245:108644. doi: 10.1016/j.vetmic.2020.108644. Epub 2020 May 11.
VetPath is an ongoing pan-European antimicrobial susceptibility monitoring programme collecting pathogens from diseased cattle, pigs and poultry not recently treated with antibiotics. Non-duplicate isolates (n = 1244) were obtained from cows with acute clinical mastitis in eight countries during 2015-2016 for centrally antimicrobial susceptibility testing according CLSI standards. Among Escherichia coli (n = 225), resistance was high to ampicillin and tetracycline, moderate to kanamycin and low to amoxicillin/clavulanic acid and cefazolin. The MIC of danofloxacin, enrofloxacin and marbofloxacin were 0.03 and 0.06 μg/mL. For Klebsiella spp. (n = 70), similar results were noted, except for ampicillin and kanamycin. We detected 3.7 % (11/295) Enterobacteriaceae isolates carrying an ESBL/AmpC gene. Staphylococcus aureus (n = 247) and coagulase-negative staphylococci (CoNS; n = 189) isolates were susceptible to most antimicrobials tested except to penicillin (25.1 and 29.1 % resistance). Two S. aureus and thirteen CoNS isolates harboured mecA gene. Streptococcus uberis isolates (n = 208) were susceptible to β-lactam antibiotics (87.1-94.7 % susceptibility), 23.9 % were resistant to erythromycin and 37.5 % to tetracycline. Resistance to pirlimycin was moderate. For Streptococcus dysgalactiae (n = 132) the latter figures were 10.6 and 43.2 %; pirlimycin resistance was low. MIC values for Streptococcus agalactiae, Trueperella pyogenes and Corynebacterium spp. were generally low. This current VetPath study shows that mastitis pathogens were susceptible to most antimicrobials with exceptions of staphylococci against penicillin and streptococci against erythromycin or tetracycline. For most antimicrobials, the percentage resistance and MIC values among the major pathogens were comparable to that of the preceeding VetPath surveys. This work highlights the high need to set additional clinical breakpoints for antimicrobials frequently used to treat mastitis.
VetPath 是一个正在进行的泛欧抗菌药物敏感性监测计划，该计划从最近未使用过抗生素治疗的患病牛、猪和家禽中收集病原体。2015-2016 年期间，从八个国家患有急性临床乳腺炎的奶牛中获得了非重复分离株（n=1244），并根据 CLSI 标准进行了中心抗菌药物敏感性测试。在 225 株大肠杆菌（Escherichia coli）中，对氨苄西林和四环素的耐药率较高，对卡那霉素的耐药率为中度，对阿莫西林/克拉维酸和头孢唑林的耐药率较低。丹氟沙星、恩诺沙星和马波沙星的 MIC 为 0.03 和 0.06μg/ml。对于克雷伯氏菌属（Klebsiella spp.）（n=70），除了氨苄西林和卡那霉素外，还观察到类似的结果。我们检测到 3.7%（11/295）的肠杆菌科分离株携带 ESBL/AmpC 基因。金黄色葡萄球菌（Staphylococcus aureus）（n=247）和凝固酶阴性葡萄球菌（CoNS；n=189）分离株对大多数测试的抗菌药物敏感，除了青霉素（25.1%和 29.1%耐药）。2 株金黄色葡萄球菌和 13 株凝固酶阴性葡萄球菌分离株携带 mecA 基因。无乳链球菌（Streptococcus uberis）分离株（n=208）对β-内酰胺类抗生素敏感（87.1-94.7%的敏感性），23.9%对红霉素耐药，37.5%对四环素耐药。对吡利霉素的耐药性为中度。对于停乳链球菌（Streptococcus dysgalactiae），后者的比例分别为 10.6%和 43.2%；吡利霉素耐药性较低。无乳链球菌、酿脓链球菌和棒状杆菌属的 MIC 值通常较低。当前的 VetPath 研究表明，乳腺炎病原体对大多数抗菌药物敏感，但金黄色葡萄球菌对青霉素耐药，链球菌对红霉素或四环素耐药。对于大多数抗菌药物，主要病原体的耐药率和 MIC 值与之前的 VetPath 调查相当。这项工作强调了非常有必要为经常用于治疗乳腺炎的抗菌药物设定额外的临床断点。
Zotero 插件