单侧急性前葡萄膜炎与泪液蛋白质组的同侧变化相关，该变化涉及肝X受体/视黄醇X受体（LXR/RXR）信号通路。
Unilateral acute anterior uveitis is associated with ipsilateral changes in the tear fluid proteome that involves the LXR/RXR pathway.
作者信息
Eidet Jon Roger, Jørstad Øystein Kalsnes, Fostad Ida G, Olstad Ole K, Sørland Ragnhild Ø, Moe Morten C, Petrovski Goran, Pepaj Milaim
机构信息
Center for Eye Research, Department of Ophthalmology, Oslo University Hospital, University of Oslo, Oslo, Norway.
Faculty of Dentistry, Department of Oral Biology, University of Oslo, Oslo, Norway.
出版信息
J Ophthalmic Inflamm Infect. 2020 May 27;10(1):13. doi: 10.1186/s12348-020-00204-4.
PURPOSE
To determine whether unilateral acute anterior uveitis (AAU) induces ipsilateral changes in the tear fluid proteome.
METHODS
Five patients (25-77 years old) with unilateral AAU were included. Tear fluid samples were obtained using Schirmer's test strips. The healthy eye served as control. Proteins were identified by liquid chromatography tandem mass spectrometry.
RESULTS
Two hundred forty-two tear fluid sample proteins were identified, of which 75 were present in at least three patients. Nine proteins were at least 1.5-fold increased, whereas eight were at least 1.5-fold decreased in tears from the diseased eye compared with the healthy eye. APOBEC3A was significantly increased (1.43-fold; P = 0.04), whereas TGM2 was significantly decreased (- 1.21-fold; P = 0.03) in tears from the diseased eye relative to the healthy eye. Ingenuity Pathway Analysis identified LXR/RXR (P < 1.02E-4) as a top canonical pathway.
CONCLUSION
Unilateral AAU induced detectable changes in the ipsilateral tear fluid proteome and involvement of the inflammation-associated LXR/RXR pathway.
目的
确定单侧急性前葡萄膜炎（AAU）是否会引起同侧泪液蛋白质组的变化。
方法
纳入5例（年龄25 - 77岁）单侧AAU患者。使用泪液试纸条获取泪液样本。健侧眼作为对照。通过液相色谱串联质谱法鉴定蛋白质。
结果
共鉴定出242种泪液样本蛋白质，其中75种在至少3例患者中存在。与健侧眼相比，患侧眼中9种蛋白质增加至少1.5倍，而8种蛋白质减少至少1.5倍。相对于健侧眼，患侧眼中载脂蛋白B mRNA编辑酶催化多肽样蛋白3A（APOBEC3A）显著增加（1.43倍；P = 0.04），而转谷氨酰胺酶2（TGM2）显著减少（-1.21倍；P = 0.03）。通路分析识别出肝X受体/视黄醇X受体（LXR/RXR）（P < 1.02E - 4）为首要经典通路。
结论
单侧AAU引起同侧泪液蛋白质组可检测到的变化，并涉及炎症相关的LXR/RXR通路。
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