Irie S, Minguell J J, Tavassoli M
University of Mississippi School of Medicine, Jackson 39216.
Biochem Int. 1988 Dec;17(6):1079-85.
To investigate the microheterogeneity of the glycan chain of rat transferrin, either the protein moiety was labeled with 125I or the sialyl residues with 3H. The molecule was then subjected to Con A chromatography. Three components were obtained. Each was enzymatically desialylated and sialyl/protein molar ratios were calculated. The native protein as well as the 3 components were also subjected to isoelectric focusing. The results indicated that rat transferrin may have 3 types of glycan chain: The major type (60%) corresponds to a molecular species with triantennary branching, while 30% consists of molecules with biantennary and 10% with tetraantennary branching. The last species has not been previously described.
为研究大鼠转铁蛋白聚糖链的微观异质性，分别用¹²⁵I标记蛋白质部分或用³H标记唾液酸残基。然后将该分子进行刀豆球蛋白A层析。得到了三个组分。对每个组分进行酶促去唾液酸化并计算唾液酸/蛋白质摩尔比。天然蛋白质以及这三个组分也进行了等电聚焦。结果表明大鼠转铁蛋白可能有三种类型的聚糖链：主要类型（60%）对应具有三分支的分子种类，而30%由具有双分支的分子组成，10%由具有四分支的分子组成。最后一种类型此前尚未有过描述。
