Efferth T, Volm M
Institute of Experimental Pathology, German Cancer Research Center, Heidelberg.
Arzneimittelforschung. 1988 Dec;38(12):1771-4.
In attempts to develop simple and rapid assays for detection of multidrug resistance (MDR) doxorubicin-resistant S180 (S180DOX), colchicine-resistant CHO (CHOCOL) and cytarabine (cytosine-arabinoside)-resistant L1210 cell lines (L1210AraC) were investigated. S180DOX and CHOCOL cells express the MDR-phenotype while L1210AraC does not. Using a previously described short-term assay firstly, inhibition of incorporation of radioactive nucleic acid precursors into tumour cells after addition of doxorubicin was measured. Secondly, the accumulation of the fluorescent dye rhodamine 123 (R123) in the different cell lines were analyzed. It could be observed that the resistant S180DOX and CHOCOL cells needed significantly more time to accumulate R123 than their sensitive parental cell lines or L1210AraC cells. Therefore, both assays are adequate tools for the rapid detection of MDR.
为了开发用于检测多药耐药（MDR）阿霉素耐药S180（S180DOX）、秋水仙碱耐药CHO（CHOCOL）和阿糖胞苷（胞嘧啶阿拉伯糖苷）耐药L1210细胞系（L1210AraC）的简单快速检测方法，对这些细胞系进行了研究。S180DOX和CHOCOL细胞表现出MDR表型，而L1210AraC则没有。首先，使用先前描述的短期检测方法，测量加入阿霉素后放射性核酸前体掺入肿瘤细胞的抑制情况。其次，分析了荧光染料罗丹明123（R123）在不同细胞系中的积累情况。可以观察到，耐药的S180DOX和CHOCOL细胞积累R123所需的时间明显比其敏感亲代细胞系或L1210AraC细胞长。因此，这两种检测方法都是快速检测MDR的合适工具。
