Moore Marc C, Bonvallet Paul P, Damaraju Sita M, Modi Heli N, Gandhi Ankur, McFetridge Peter S
Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma, USA.
Product Development, Integra LifeSciences, Princeton, New Jersey, 08540, USA.
J Biomed Mater Res B Appl Biomater. 2020 Nov;108(8):3076-3083. doi: 10.1002/jbm.b.34635. Epub 2020 May 26.
There is a growing clinical demand in the wound care market to treat chronic wounds such as diabetic foot ulcers. Advanced cell and tissue-based products (CTPs) are often used to address challenging chronic wounds where healing has stalled. These products contain active biologics such as growth factors and cytokines as well as structural components that support and stimulate cell growth and assist in tissue regeneration. This study addresses the in vitro biologic effects of a clinically available dehydrated amniotic membrane allograft (DAMA). The broad mechanism of action results from DAMA's biologic composition that leads to stimulation of cell migration cell proliferation, and reduction of pro-inflammatory cytokines. Results show that DAMA possesses growth factors and cytokines such as EGF, FGF, PDGFs, VEGF, TGF-β, IL-8, and TIMPs 1 and 2. Furthermore, in vitro experiments demonstrate that DAMA stimulates cell proliferation, cell migration, secretion of collagen type I, and the reduction of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α. This study findings are consistent with the clinical benefits previously published for DAMA and other CTPs in chronic wounds suggesting that the introduction of DAMA to non-healing, complex wounds helps to improve the wound milieu by providing essential structural components, cytokines, and growth factors to create an appropriate environment for wound healing.
伤口护理市场对治疗慢性伤口（如糖尿病足溃疡）的临床需求日益增长。先进的基于细胞和组织的产品（CTP）通常用于处理愈合停滞的具有挑战性的慢性伤口。这些产品含有活性生物制剂，如生长因子和细胞因子，以及支持和刺激细胞生长并协助组织再生的结构成分。本研究探讨了一种临床可用的脱水羊膜同种异体移植物（DAMA）的体外生物学效应。DAMA的生物学组成导致其广泛的作用机制，从而刺激细胞迁移、细胞增殖并减少促炎细胞因子。结果表明，DAMA含有生长因子和细胞因子，如表皮生长因子（EGF）、成纤维细胞生长因子（FGF）、血小板衍生生长因子（PDGFs）、血管内皮生长因子（VEGF）、转化生长因子-β（TGF-β）、白细胞介素-8（IL-8）以及基质金属蛋白酶组织抑制因子1和2（TIMPs 1和2）。此外，体外实验表明，DAMA可刺激细胞增殖、细胞迁移、I型胶原蛋白的分泌，并减少促炎细胞因子白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）。本研究结果与先前发表的关于DAMA和其他CTP在慢性伤口中的临床益处一致，表明将DAMA引入不愈合的复杂伤口有助于通过提供必需的结构成分、细胞因子和生长因子来改善伤口环境，从而为伤口愈合创造适宜的环境。
