Zhang Shang, Gao Lichang, Wang Pin, Ma Yuyan, Wang Xiaoliang, Wen Jie, Cheng Yu, Liu Changlin, Zhang Chunxia, Liu Changfeng, Yan Yongli, Zhao Chengru
Success Bio-Tech Co., Ltd., Biomedical Material Engineering Laboratory of Shandong Province, Jinan, China.
Department of Gynecology and Obstetrics, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.
Front Bioeng Biotechnol. 2022 Aug 11;10:952498. doi: 10.3389/fbioe.2022.952498. eCollection 2022.
Allogeneic amnion tissues have been widely used in tissue repair and regeneration, especially a remarkable trend of clinical uses in chronic wound repair. The virus inactivation procedures are necessary and required to be verified for the clinical use and approval of biological products. Cobalt-60 (Co-60) or electron-beam (e-beam) is the common procedure for virus and bacterial reduction, but the excessive dose of irradiation was reported to be harmful to biological products. Herein, we present a riboflavin (RB)-ultraviolet light (UV) method for virus inactivation of amnion and chorion tissues. We used the standard limiting dilution assay to test the viral reduction capacity of the RB-UV method on amnion or chorion tissues loaded with four types of model viruses. We found RB-UV was a very effective procedure for inactivating viruses of amnion and chorion tissues, which could be used as a complementary method to Co-60 irradiation. In addition, we also screened the washing solutions and drying methods for the retention of growth factors.
异体羊膜组织已广泛应用于组织修复和再生，尤其是在慢性伤口修复方面呈现出显著的临床应用趋势。病毒灭活程序对于生物制品的临床使用和批准而言是必要且需验证的。钴-60（Co-60）或电子束（e-beam）是常见的病毒和细菌减量程序，但据报道，过高剂量的辐照对生物制品有害。在此，我们提出一种核黄素（RB）-紫外线（UV）方法用于羊膜和绒毛膜组织的病毒灭活。我们使用标准的有限稀释试验来测试RB-UV方法对负载四种模型病毒的羊膜或绒毛膜组织的病毒减量能力。我们发现RB-UV是一种非常有效的灭活羊膜和绒毛膜组织病毒的程序，可作为Co-60辐照的补充方法。此外，我们还筛选了用于保留生长因子的洗涤溶液和干燥方法。
