Feng Jin, Huang Meigui, Chai Zhi, Li Chunyang, Huang Wuyang, Cui Li, Li Ying
Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing 210014, China.
Department of food science and engineering, College of light industry and food engineering, Nanjing forestry university, 159 Longpan Road, Nanjing 210037, China.
Food Funct. 2020 Jun 24;11(6):5223-5239. doi: 10.1039/d0fo00473a.
In this study, the influences of liquid medium-chain triglyceride (MCT) and solid glyceryl tristearate (GTS) contents in the lipid matrix of nanostructured lipid carriers (NLCs) on their delivering capacities with respect to curcumin (Cur) were investigated by using a simulated gastrointestinal tract and Caco-2 monolayer models. The transformation of the encapsulated Cur decreased on increasing the MCT content in the lipid matrix of NLCs because it facilitated their lipolysis and promoted the exposure of Cur to a harsher exterior environment. Cur bioaccessibility was positively correlated with the level of micellized stearic acid resulting from GTS hydrolysis, which might be attributed to the fact that it could afford large hydrophobic domains to accommodate Cur. This value initially increased with an increase in the MCT content, reaching a maximum at 20% (w/w) and decreasing thereafter. The intestinal absorption of micellar Cur ranged from 26.06% to 38.76%, and a majority of the transported molecules were its reductive and conjugative metabolites. Overall, NLC containing 20% MCT in the lipid matrix afforded the highest Cur bioavailability, followed by that containing 10, 0, 40, 60, and 100% MCT. This work provides useful insights into the rational design of NLCs to optimize the bioavailability of the loaded agent.
在本研究中，通过使用模拟胃肠道和Caco-2单层模型，研究了纳米结构脂质载体（NLCs）脂质基质中液体中链甘油三酯（MCT）和固体硬脂酸甘油酯（GTS）含量对其姜黄素（Cur）递送能力的影响。随着NLCs脂质基质中MCT含量的增加，包封的Cur的转化率降低，因为它促进了它们的脂解作用，并促进了Cur暴露于更恶劣的外部环境。Cur的生物可及性与GTS水解产生的胶束化硬脂酸水平呈正相关，这可能归因于它可以提供大的疏水区域来容纳Cur。该值最初随着MCT含量的增加而增加，在20%（w/w）时达到最大值，此后降低。胶束化Cur的肠道吸收范围为26.06%至38.76%，并且大多数转运分子是其还原和共轭代谢物。总体而言，脂质基质中含有20% MCT的NLC提供了最高的Cur生物利用度，其次是含有10%、0%、40%、60%和100% MCT的NLC。这项工作为合理设计NLCs以优化负载剂的生物利用度提供了有用的见解。
