姜黄素衍生物MTH-3调节棕榈酸酯诱导的小鼠成肌细胞CC细胞中的胰岛素抵抗。
Curcumin Derivative MTH-3 Regulates Palmitate-induced Insulin Resistance in Mouse Myoblast CC Cells.
作者信息
Chiu Yu-Jen, Lo Yu-Hsiang, Yang Jai-Sing, Kuo Sheng-Chu, Tsai Shih-Chang
机构信息
Division of Plastic and Reconstructive Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei, Taiwan, R.O.C.
Department of Surgery, School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan, R.O.C.
出版信息
In Vivo. 2021 Nov-Dec;35(6):3181-3191. doi: 10.21873/invivo.12613.
BACKGROUND/AIM: At present, there are no effective drugs for the treatment of insulin resistance. MTH-3, a curcumin derivative, exerts potent anti-cancer effects. The aim of the present study was to explore whether MTH-3 is capable of regulating palmitic acid (PA)-induced insulin resistance in CC cells.
MATERIALS AND METHODS
Cell viability was examined using the MTT assay. CC cells were treated with PA and evaluated for the production of oil droplets using an Oil Red O assay. Glucose uptake was analysed by the 2-NBDG assay.
RESULTS
Treatment of cells with up to 500 μM PA for 24 h or with 5 or 10 μM MTH-3 had no effect on cell viability. PA induced production of oil droplets in CC cells. After adding MTH-3, the quantity of oil droplets decreased significantly and glucose uptake recovered.
CONCLUSION
MTH-3 may become an efficient drug for the treatment of insulin resistance and associated diseases.
背景/目的：目前，尚无治疗胰岛素抵抗的有效药物。姜黄素衍生物MTH-3具有强大的抗癌作用。本研究的目的是探讨MTH-3是否能够调节棕榈酸（PA）诱导的CC细胞胰岛素抵抗。
材料与方法
采用MTT法检测细胞活力。用PA处理CC细胞，并使用油红O法评估油滴的产生。通过2-NBDG法分析葡萄糖摄取。
结果
用高达500μM的PA处理细胞24小时或用5或10μM的MTH-3处理对细胞活力没有影响。PA诱导CC细胞中油滴的产生。添加MTH-3后，油滴数量显著减少，葡萄糖摄取恢复。
结论
MTH-3可能成为治疗胰岛素抵抗及相关疾病的有效药物。
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