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建立并鉴定一种新型的“双打击”滤泡性淋巴瘤细胞系，FL-SJC。

Establishment and characterization of a novel 'double-hit' follicular lymphoma cell line, FL-SJC.

机构信息

Affiliated Hospital of Jiangsu University, Zhenjiang, China.

Jiangdu People's Hospital, Yangzhou, China.

出版信息

J Cell Mol Med. 2020 Jul;24(14):7928-7938. doi: 10.1111/jcmm.15425. Epub 2020 May 27.

DOI:10.1111/jcmm.15425
PMID:32459397
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7348184/
Abstract

About 5 per cent of follicular lymphoma (FL) cases are double-hit (DH) lymphomas. Double-hit follicular lymphoma (DHFL) cell lines can improve our understanding and drug development on FL. But there are only few DHFL cell lines. Here, we established a new MYC/BCL2 DHFL cell line, FL-SJC. The cells were obtained from the hydrothorax of a patient with MYC/BCL2 DHFL and cultured for 140 passages in vitro. FL-SJC cells demonstrated CD19 , CD20 , CD22 , HLA-DR , CD10 , CD38 , Lambda CD23 , CD5 and Kappa . The chromosome karyotypic analysis confirmed the co-existence of t(8;22)(q24;q11) and t(14;18)(q32;q21), as well as additional abnormalities involving chromosomes 2 and 3. Fluorescence in situ hybridization analysis (FISH) showed IGH/BCL2 fusion gene and the MYC rearrangement. In addition, the FL-SJC cells displayed KMT2D/MLL2 and CREBBP gene mutations. After subcutaneous inoculation of FL-SJC cells, the SCID mice developed solid tumour masses within 6-8 weeks. FL-SJC cells were proven to be free of Epstein-Barr (EB) virus infection and be multidrug-resistant. In a conclusion, the FL-SJC cell line has been identified as a novel MYC/BCL2 double-hit follicular lymphoma that can be used as a potentially available tool for the clinical and basic research, together with the drug development for MYC/BCL2 DHFL.

摘要

大约 5%的滤泡性淋巴瘤 (FL) 病例为双打击 (DH) 淋巴瘤。双打击滤泡性淋巴瘤 (DHFL) 细胞系可以帮助我们更好地理解和开发 FL 药物。但是，DHFL 细胞系非常少。在这里，我们建立了一种新的 MYC/BCL2 DHFL 细胞系，FL-SJC。该细胞系源自一名 MYC/BCL2 DHFL 患者的胸腔积液，经过 140 代体外培养。FL-SJC 细胞表达 CD19、CD20、CD22、HLA-DR、CD10、CD38、Lambda CD23、CD5 和 Kappa。染色体核型分析证实了 t(8;22)(q24;q11)和 t(14;18)(q32;q21)的共存，以及涉及染色体 2 和 3 的额外异常。荧光原位杂交分析 (FISH) 显示IGH/BCL2 融合基因和 MYC 重排。此外，FL-SJC 细胞还显示 KMT2D/MLL2 和 CREBBP 基因突变。将 FL-SJC 细胞皮下接种后，SCID 小鼠在 6-8 周内形成实体瘤。FL-SJC 细胞被证明未感染 Epstein-Barr (EB) 病毒，且对多种药物具有耐药性。总之，该 FL-SJC 细胞系已被鉴定为一种新型的 MYC/BCL2 双打击滤泡性淋巴瘤，可作为一种潜在的临床和基础研究工具，以及用于 MYC/BCL2 DHFL 的药物开发。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b6/7348184/2f846d809760/JCMM-24-7928-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b6/7348184/ff80bef3ce8b/JCMM-24-7928-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b6/7348184/6727c4c780b3/JCMM-24-7928-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b6/7348184/bf80c53d2fd1/JCMM-24-7928-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b6/7348184/1c6309f32816/JCMM-24-7928-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b6/7348184/2f846d809760/JCMM-24-7928-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b6/7348184/ff80bef3ce8b/JCMM-24-7928-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b6/7348184/6727c4c780b3/JCMM-24-7928-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b6/7348184/bf80c53d2fd1/JCMM-24-7928-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b6/7348184/1c6309f32816/JCMM-24-7928-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2b6/7348184/2f846d809760/JCMM-24-7928-g005.jpg

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