Department of Surgery, University of California, Irvine , Orange, CA, USA.
Department of Pediatrics, Pediatric Exercise and Genomics Research Center, University of California, Irvine , Irvine, CA, USA.
Islets. 2020 May 3;12(3):41-58. doi: 10.1080/19382014.2020.1750933. Epub 2020 May 27.
The use of pancreata from pre-weaned piglets has the potential to serve as an unlimited alternative source of islets for clinical xenotransplantation. As pre-weaned porcine islets (PPIs) are immature and require prolonged culture, we developed an islet maturation media (IMM) and evaluated its effect on improving the quantity and quality of PPIs over 14 days of culture.
PPIs were isolated from the pancreata of pre-weaned Yorkshire piglets (8-15 days old). Each independent islet isolation was divided for culture in either control Ham's F-10 media (n = 5) or IMM (n = 5) for 14 days. On day 3, 7 and 14 of culture, islets were assessed for islet yield, isolation index, viability, insulin content, endocrine cellular composition, differentiation of beta cells, and insulin secretion during glucose stimulation.
In comparison to control islets, culturing PPIs in IMM significantly increased islet yield. PPIs cultured in IMM also maintained a stable isolation index and viability throughout 14 days of culture. The insulin content, endocrine cellular composition, and differentiation of beta cells were significantly improved in PPIs cultured in IMM, which subsequently augmented their insulin secretory capacity in response to glucose challenge compared to control islets.
Culturing PPIs in IMM increases islet yield, isolation index, viability, insulin content, endocrine cellular composition, differentiation of endocrine progenitor cells toward beta cells, and insulin secretion. Due to the improved islet quantity and quality after culture, the use of IMM in the culture of PPIs will assist to advance the outcomes of clinical islet xenotransplantation.
使用未断奶仔猪的胰腺有可能成为临床异种移植胰岛的无限替代来源。由于未断奶猪胰岛（PPIs）不成熟，需要长时间培养，我们开发了胰岛成熟培养基（IMM），并评估了其在 14 天培养过程中提高 PPIs 数量和质量的效果。
从未断奶约克夏仔猪（8-15 天大）的胰腺中分离 PPIs。每个独立的胰岛分离物被分为在对照 Ham's F-10 培养基（n=5）或 IMM（n=5）中培养 14 天。在培养的第 3、7 和 14 天，评估胰岛的胰岛产量、分离指数、活力、胰岛素含量、内分泌细胞组成、β细胞分化和葡萄糖刺激下的胰岛素分泌。
与对照胰岛相比，在 IMM 中培养 PPIs 显著增加了胰岛产量。在 14 天的培养过程中，PPIs 在 IMM 中培养也保持了稳定的分离指数和活力。IMM 中培养的 PPIs 的胰岛素含量、内分泌细胞组成和β细胞分化明显改善，随后与对照胰岛相比，它们对葡萄糖刺激的胰岛素分泌能力增强。
在 IMM 中培养 PPIs 可增加胰岛产量、分离指数、活力、胰岛素含量、内分泌细胞组成、内分泌祖细胞向β细胞的分化以及胰岛素分泌。由于培养后胰岛的数量和质量得到改善，IMM 在 PPIs 培养中的应用将有助于推进临床胰岛异种移植的结果。
