Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
Department of Microbiology, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
Biomed Pharmacother. 2020 Aug;128:110120. doi: 10.1016/j.biopha.2020.110120. Epub 2020 May 24.
BACKGROUND: Salvia officinalis L. (Lamiaceae) is known to have antibacterial properties possibly conducive to the healing process of infected wounds. PURPOSE: The present study aimed to evaluate the effects of an ointment containing Salvia officinalis essential oil (SOO) on an infected wound model. METHODS: Essential oil hydrodistillated from the dried leaves of the plant was analyzed by GC-FID and GC-MS. After creating two full-thickness cutaneous wounds, mice were classified into four groups, control, and animals treated with 2 % mupirocin® (standard positive drug), and 2 % and 4 % (w/w) of SOO. In order to evaluate the effects of SOO on the wound healing phases, the expression levels of interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), cyclin-D1, Bcl-2, fibroblast growth factor-2 (FGF-2) and vascular endothelial growth factors (VEGF) were analyzed using qRT-PCR. Immunohistochemistry analysis, tissue total antioxidant capacity (TAC) and malondialdehyde (MDA) were further assessed in all groups. RESULTS: Concerning essential oil, the main compounds were found to be cis-thujone (26.8 %), camphor (16.4 %), trans-thujone (14.1 %) and 1,8-cineole (10.8 %). Our findings showed that the topical application of SOO was able to shorten the inflammatory phase and accelerate the cellular proliferation, re-vascularization, collagen deposition and re-epithelialization in comparison to the control group (p < 0.05). Moreover, increased mRNA levels of FGF-2 and VEGF, and up-regulation of cyclin-D1 and Bcl-2 were observed following the topical application of SOO compared to the control group (p < 0.05). The expression levels of IL-6, IL-1β and TNF-α were reduced in animals treated with SOO on days 3, 7 and 14 (p < 0.05). CONCLUSIONS: Administration of SOO increased the TAC level and reduced the MDA content and levels of IL-1β and TNF-α. It is concluded that SOO is able to accelerate the wound healing process by regulating the expression of pro-inflammatory cytokines, growth factors, and antioxidant properties.
背景：鼠尾草（唇形科）具有抗菌特性，可能有助于感染伤口的愈合过程。
目的：本研究旨在评估含有鼠尾草精油（SOO）的软膏对感染伤口模型的影响。
方法：对植物干燥叶片进行水蒸气蒸馏得到精油，采用 GC-FID 和 GC-MS 进行分析。在创建两个全层皮肤伤口后，将小鼠分为四组：对照组和用 2%莫匹罗星（标准阳性药物）、2%和 4%（w/w）SOO 治疗的动物。为了评估 SOO 对伤口愈合阶段的影响，使用 qRT-PCR 分析白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、细胞周期蛋白 D1、Bcl-2、成纤维细胞生长因子-2（FGF-2）和血管内皮生长因子（VEGF）的表达水平。进一步对所有组进行免疫组织化学分析、组织总抗氧化能力（TAC）和丙二醛（MDA）评估。
结果：关于精油，发现主要化合物为顺式-侧柏酮（26.8%）、樟脑（16.4%）、反式-侧柏酮（14.1%）和 1,8-桉叶油醇（10.8%）。与对照组相比，SOO 的局部应用能够缩短炎症期并加速细胞增殖、再血管化、胶原沉积和再上皮化（p<0.05）。此外，与对照组相比，SOO 局部应用后观察到 FGF-2 和 VEGF 的 mRNA 水平升高，以及细胞周期蛋白 D1 和 Bcl-2 的上调（p<0.05）。在第 3、7 和 14 天用 SOO 治疗的动物中，IL-6、IL-1β 和 TNF-α 的表达水平降低（p<0.05）。
结论：SOO 给药增加了 TAC 水平，降低了 MDA 含量以及 IL-1β 和 TNF-α 的水平。结论是 SOO 通过调节促炎细胞因子、生长因子和抗氧化特性来加速伤口愈合过程。
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