植物孤种代谢途径重建的流水线。

A Pipeline for Metabolic Pathway Reconstruction in Plant Orphan Species.

机构信息

Plant Physiology, Department of Organisms and Systems Biology, University Institute of Biotechnology of Asturias (IUBA), University of Oviedo, Oviedo, Asturias, Spain.

Agroforestry and Plant Biochemistry, Proteomics and Systems Biology, Department of Biochemistry and Molecular Biology, University of Cordoba, UCO-CeiA3, Cordoba, Spain.

出版信息

Methods Mol Biol. 2020;2139:367-380. doi: 10.1007/978-1-0716-0528-8_27.

DOI:10.1007/978-1-0716-0528-8_27

PMID:32462600

Abstract

In the era of high-throughput biology, it is necessary to develop a simple pipeline for metabolic pathway reconstruction in plant orphan species. However, obtaining a global picture of the plant metabolism may be challenging, especially in nonmodel species. Moreover, the use of bioinformatics tools and statistical analyses is required. This chapter describes how to use different software and online tools for the reconstruction of metabolic pathways of plant species using existing pathway knowledge. In particular, Quercus ilex omics data is employed to develop the present pipeline.

摘要

在高通量生物学时代，有必要为植物孤儿物种的代谢途径重建开发一个简单的流程。然而，获得植物代谢的全貌可能具有挑战性，特别是在非模式物种中。此外，需要使用生物信息学工具和统计分析。本章描述了如何使用不同的软件和在线工具，利用现有的途径知识来重建植物物种的代谢途径。特别是，使用栗树的组学数据来开发当前的流程。