Department of Biochemistry and Molecular Biology, Agrifood Campus of International Excellence, University of Cordoba, Carretera Nacional IV, km 396, 14014 Córdoba, Spain.
Departamento de Fitoquímica, Dirección de Investigación de la Facultad de Ciencias Químicas de la Universidad Nacional de Asunción, Asunción 1001-1925, Paraguay.
Int J Mol Sci. 2019 Feb 6;20(3):692. doi: 10.3390/ijms20030692.
Proteomics has had a big impact on plant biology, considered as a valuable tool for several forest species, such as , , , and . This review assesses the potential and limitations of the proteomics approaches and is focused on as a model species and other forest tree species. Proteomics has been used with since 2003 with the main aim of examining natural variability, developmental processes, and responses to biotic and abiotic stresses as in other species of the genus or . As with the progress in techniques in proteomics in other plant species, the research in moved from 2-DE based strategy to the latest gel-free shotgun workflows. Experimental design, protein extraction, mass spectrometric analysis, confidence levels of qualitative and quantitative proteomics data, and their interpretation are a true challenge with relation to forest tree species due to their extreme orphan and recalcitrant (non-orthodox) nature. Implementing a systems biology approach, it is time to validate proteomics data using complementary techniques and integrate it with the -omics and classical approaches. The full potential of the protein field in plant research is quite far from being entirely exploited. However, despite the methodological limitations present in proteomics, there is no doubt that this discipline has contributed to deeper knowledge of plant biology and, currently, is increasingly employed for translational purposes.
蛋白质组学对植物生物学产生了重大影响，被认为是几种森林物种的有价值的工具，如 、 、 、 。本综述评估了蛋白质组学方法的潜力和局限性，并侧重于 作为模式物种和其他森林树种。自 2003 年以来，蛋白质组学已用于 ，主要目的是研究自然变异性、发育过程以及对生物和非生物胁迫的反应，如属中的其他物种 或 。与其他植物物种中蛋白质组学技术的进展一样， 在蛋白质组学方面的研究从基于 2-DE 的策略转向了最新的无凝胶 shotgun 工作流程。由于其极端的孤儿和顽固（非正统）性质，实验设计、蛋白质提取、质谱分析、定性和定量蛋白质组学数据的置信水平及其解释对森林树种来说是一个真正的挑战。实施系统生物学方法，现在是时候使用互补技术验证蛋白质组学数据并将其与 -omics 和经典方法整合起来了。蛋白质组学在植物研究中的全部潜力还远远没有得到充分利用。然而，尽管蛋白质组学存在方法学上的局限性，但毫无疑问，该学科已经为更深入地了解植物生物学做出了贡献，并且目前越来越多地用于转化目的。
