Division of Clinical Neuroscience, Chiba University Center for Forensic Mental Health, Chiba, Japan.
Innovation Division, Kagome Co., Ltd., Nasushiobara, Japan.
Neuropsychopharmacol Rep. 2020 Sep;40(3):268-274. doi: 10.1002/npr2.12112. Epub 2020 May 28.
Epidemiological data suggest that maternal immune activation (MIA) plays a role in the etiology of neuropsychiatric disorders including autism spectrum disorder (ASD) and schizophrenia. However, there is no prophylactic nutrition that can prevent the onset of neurodevelopmental disorders in offspring after MIA. The aim of this study was undertaken to examine whether dietary intake of glucoraphanin (GF: the precursor of a natural anti-inflammatory compound sulforaphane) can prevent the onset of behavioral abnormalities in offspring after MIA.
One percent of GF food pellet or normal food pellet was given into female mice during pregnancy and lactation (from E5 to P21). Saline (5 mL/kg/d) or poly(I:C) (5 mg/kg/d) was injected into pregnant mice from E12 to E17. Behavioral tests and immunohistochemistry of parvalbumin (PV) were performed in male offspring.
Dietary intake of GF during pregnancy and lactation prevented cognitive deficits and social interaction deficits in the juvenile offspring after MIA. Furthermore, dietary intake of GF during pregnancy and lactation prevented cognitive deficits in the adult offspring after MIA. Moreover, dietary intake of GF prevented the reduction of PV immunoreactivity in the medial prefrontal cortex of adult offspring after MIA.
These data suggest that dietary intake of GF during pregnancy and lactation could prevent behavioral abnormalities in offspring after MIA.
流行病学数据表明，母体免疫激活（MIA）在神经精神疾病的发病机制中起作用，包括自闭症谱系障碍（ASD）和精神分裂症。然而，没有预防营养可以预防 MIA 后后代神经发育障碍的发生。本研究旨在探讨母体在妊娠和哺乳期摄入萝卜硫素（GF：天然抗炎化合物萝卜硫烷的前体）是否可以预防 MIA 后后代行为异常的发生。
在妊娠和哺乳期（从 E5 到 P21），将 1%的 GF 食物颗粒或正常食物颗粒给予雌性小鼠。从 E12 到 E17 给怀孕的老鼠注射盐水（5 mL/kg/d）或聚 I:C（5 mg/kg/d）。对雄性后代进行行为测试和钙结合蛋白 Parvalbumin（PV）免疫组织化学分析。
妊娠和哺乳期 GF 的饮食摄入可预防 MIA 后幼鼠的认知缺陷和社交互动缺陷。此外，妊娠和哺乳期 GF 的饮食摄入可预防 MIA 后成年后代的认知缺陷。此外，GF 的饮食摄入可预防 MIA 后成年后代内侧前额叶皮质中 PV 免疫反应性的降低。
这些数据表明，妊娠和哺乳期 GF 的饮食摄入可以预防 MIA 后后代的行为异常。
