Xiangya International Academy of Translational Medicine at Central South University, Changsha, 410013, Hunan, China.
Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.
Org Lett. 2020 Jun 19;22(12):4614-4619. doi: 10.1021/acs.orglett.0c01224. Epub 2020 May 28.
Bohemamines (BHMs) are bacterial alkaloids containing a pyrrolizidine core with two unusual methyl groups. Herein we report the activation of BHMs biosynthesis using a ribosome engineering approach. Characterization of the gene cluster reveals that nonribosomal peptide synthetase BhmJ and Baeyer-Villiger monooxygenase BhmK are responsible for the formation of the pyrrolizidine core, which is further methylated on C-7 by methyltransferase BhmG. The 9-methyl group of BHMs is instead originated from a nonproteinogenic amino acid (2,5)-5-methylproline.
Bohemamines (BHMs) 是一类含有吡咯里西啶核心并带有两个特殊甲基的细菌生物碱。在此，我们报道了一种核糖体工程方法来激活 BHMs 生物合成。基因簇的特征表明非核糖体肽合成酶 BhmJ 和 Baeyer-Villiger 单加氧酶 BhmK 负责吡咯里西啶核心的形成，该核心在 C-7 位进一步由甲基转移酶 BhmG 甲基化。BHMs 的 9-甲基则来源于非蛋白氨基酸（2,5）-5-甲基脯氨酸。
