School of Medicine, Zhejiang University, Hangzhou, China.
College of Basic Medical Sciences, Second Military Medical University, Shanghai, China.
Aging (Albany NY). 2020 May 28;12(10):8953-8967. doi: 10.18632/aging.103125.
Chitosan nanoparticles have been recognized as a new type of biomaterials for treatment of spinal cord injury (SCI). To develop a novel treatment method targeted delivery injured spinal cord, valproic acid labeled chitosan nanoparticles (VA-CN) were constructed and evaluated in the treatment of SCI. Our results demonstrated that administration of VA-CN significantly promoted the recovery of the function and tissue repair after SCI. Moreover, we found treatment of VA-CN inhibited the reactive astrocytes after SCI. Furthermore, administration of VA-CN enhanced immunoreactions of neuronal related marker NF160, which suggested that VA-CN could promote the neuroprotective function in rats of SCI. The production of IL-1β, IL-6 and TNF-α were significantly decreased following treatment of VA-CN. Meanwhile, administration of VA-CN effectively improved the blood spinal cord barrier (BSCB) disruption after SCI. Administration of VA-CN could enhance the recovery of neuronal injury, suppress the reactive astrocytes and inflammation, and improve the blood spinal cord barrier disruption after SCI in rats. These results provided a novel and promising therapeutic manner for SCI.
壳聚糖纳米粒已被认为是一种新型的生物材料，可用于治疗脊髓损伤（SCI）。为了开发一种针对损伤脊髓的靶向递药治疗方法，构建了载缬草酸的壳聚糖纳米粒（VA-CN），并对其在 SCI 治疗中的作用进行了评价。我们的结果表明，VA-CN 的给药显著促进了 SCI 后的功能恢复和组织修复。此外，我们发现 VA-CN 治疗抑制了 SCI 后的反应性星形胶质细胞。此外，VA-CN 的给药增强了神经元相关标志物 NF160 的免疫反应，提示 VA-CN 可促进 SCI 大鼠的神经保护功能。VA-CN 治疗后，IL-1β、IL-6 和 TNF-α 的产生明显减少。同时，VA-CN 的给药可有效改善 SCI 后的血脊髓屏障（BSCB）破坏。VA-CN 的给药可增强神经元损伤的恢复，抑制反应性星形胶质细胞和炎症，并改善 SCI 后血脊髓屏障的破坏，为 SCI 提供了一种新的有前景的治疗方法。
