Xu Danyun, Lu Jing, Wang Yuanyuan, Keerio Aafaque Ahmed, Zheng Liesheng, Chen Liguo, Ma Aimin
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R. China.
Int J Med Mushrooms. 2019;21(12):1193-1205. doi: 10.1615/IntJMedMushrooms.2019032886.
Lectins, one of the most important bioactive compounds, are nonimmunoglobulin proteins that can bind carbohydrates specifically. However, few reports have been published on Pleurotus ostreatus lectin at the molecular level. Hence, in this study, seven lectins were identified based on transcriptomes in four developmental stages, i.e., mycelium, primordium, young fruiting body, and mature fruiting body. The expression profiles of the lectin genes were verified by quantitative real-time PCR. Lectin2-lectin6 had the highest expression in mycelium, while lectin1 was rich in mature fruiting body, and lectin7 was in primordium. We inferred that lectin2-lectin6 may take part in cell flocculation, lectin7 was the critical gene for primordium formation, and lectinl may be involved in fruiting body maturation, respectively. By in silico analysis, all lectins were divided into three distinct groups. Lectin1-Lectin5 were about 38.5-40.7 kDa as extracellular protein and belonged to the PCL-like lectins. Lectin6 (15.4 kDa) was predicted in nucleus and belonged to fungal fruit body lectins. Lectin7 (38.5 kDa) was a member of legume-like lectins and located in the plasma membrane. This study will help us understand how lectins mediate mushroom development.
凝集素是最重要的生物活性化合物之一，是能够特异性结合碳水化合物的非免疫球蛋白蛋白质。然而，关于平菇凝集素在分子水平上的报道很少。因此，在本研究中，基于四个发育阶段（即菌丝体、原基、幼嫩子实体和成熟子实体）的转录组鉴定出了七种凝集素。通过实时定量PCR验证了凝集素基因的表达谱。凝集素2-凝集素6在菌丝体中表达最高，而凝集素1在成熟子实体中含量丰富，凝集素7在原基中含量丰富。我们推测凝集素2-凝集素6可能参与细胞絮凝，凝集素7是原基形成的关键基因，而凝集素1可能分别参与子实体成熟。通过电子分析，所有凝集素被分为三个不同的组。凝集素1-凝集素5作为细胞外蛋白约为38.5-40.7 kDa，属于类PCL凝集素。凝集素6（15.4 kDa）被预测存在于细胞核中，属于真菌子实体凝集素。凝集素7（38.5 kDa）是豆科样凝集素的成员，位于质膜上。这项研究将有助于我们了解凝集素如何介导蘑菇发育。
