School of Bioengineering, Dalian University of Technology, Dalian 116024, China.
Microbial Research Institute of Liaoning Province, Chaoyang 122000, China.
Int J Mol Sci. 2019 Dec 14;20(24):6317. doi: 10.3390/ijms20246317.
is a widely cultivated edible fungus around the world. At present, studies on the developmental process of the fruiting body are limited. In our study, we compared the differentially expressed proteins (DEPs) in the stipe and cap of the fruiting body by high-throughput proteomics. GO and pathway analysis revealed the great differences in the metabolic levels, including sucrose and starch metabolism, and sphingolipid signaling and metabolism, and the differences of 16 important DEPs were validated further by qPCR analysis in expression level. In order to control the cap and stipe development, several chemical inducers were applied to the primordium of the fruiting body according to the pathway enrichment results. We found that CaCl can affect the primordium differentiation through inhibiting the stipe development. EGTA (ethyleneglycol bis (β-aminoethyl ether)-N,N,N',N'-tetraacetic acid) treatment confirmed the inhibitory role of Ca in the stipe development. Our study not only shows great metabolic differences during the cap and stipe development but also reveals the underlying mechanism directing the primordium differentiation in the early development of the fruiting body for the first time. Most importantly, we provide a reliable application strategy for the cultivation and improvement of the , which can be an example and reference for a more edible fungus.
是一种在全世界广泛种植的可食用真菌。目前，关于其子实体发育过程的研究还很有限。在我们的研究中，我们通过高通量蛋白质组学比较了子实体菌柄和菌盖中的差异表达蛋白（DEPs）。GO 和通路分析显示，在代谢水平上存在很大差异，包括蔗糖和淀粉代谢、鞘脂信号和代谢，并且通过 qPCR 分析进一步验证了 16 个重要 DEPs 在表达水平上的差异。为了控制菌盖和菌柄的发育，根据通路富集结果，向子实体原基施加了几种化学诱导剂。我们发现 CaCl 可以通过抑制菌柄发育来影响原基的分化。EGTA（乙二醇双（β-氨基乙基醚）-N，N，N'，N'-四乙酸）处理证实了 Ca 在菌柄发育中的抑制作用。我们的研究不仅显示了菌盖和菌柄发育过程中的巨大代谢差异，而且首次揭示了在子实体早期发育过程中指导原基分化的潜在机制。最重要的是，我们为 的栽培和改良提供了可靠的应用策略，可为更多的可食用真菌提供范例和参考。
