School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur 613 401, Tamil Nadu, India.
School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur 613 401, Tamil Nadu, India.
Int J Biol Macromol. 2020 Oct 1;160:964-970. doi: 10.1016/j.ijbiomac.2020.05.185. Epub 2020 May 25.
The carboxymethylated inulin (CMI) nanoparticles prepared by the salt out method was demonstrated to harvest cellulolytic enzymes (Ez) directly from the clarified fermented broth of Trichoderma harzanium BPGF1. The formation of CMI nanoparticles and entrapment of Ez in CMI was confirmed by scanning electron microscopy and Fourier transform infrared spectroscopy, respectively. A factorial design was developed to maximize enzymes recovery directly from the fermented broth. A maximum of 71.68 ± 8.61% cellulolytic enzymes was recovered using 20 mg/L inulin, 2 M sodium chloroacetate at 80 °C for 2 h. The resultant CMIEz nanohybrid displayed excellent activity in broad pH and temperature. Moreover, CMIEz was reusable for >30 cycles without losing efficiency. The real-time application of CMIEz was demonstrated by hydrolyzing acid pretreated corncob. High-pressure liquid chromatography revealed that the hydrolyzed corncob contained cellobiose, glucose, galactose, xylose, mannose, and arabinose. The results highlight that carbohydrate nanoparticles was useful in engulfing enzymes directly from the fermentation broth.
采用盐析法制备的羧甲基化菊粉（CMI）纳米颗粒被证明可以直接从哈茨木霉 BPGF1 澄清发酵液中收获纤维素酶（Ez）。扫描电子显微镜和傅里叶变换红外光谱分别证实了 CMI 纳米颗粒的形成和 Ez 在 CMI 中的包埋。通过析因设计来最大限度地从发酵液中直接回收酶。使用 20mg/L 菊粉、2M 氯乙酸钠在 80°C 下反应 2 小时，可最大程度地回收 71.68±8.61%的纤维素酶。所得的 CMIEz 纳米杂化物在较宽的 pH 和温度范围内显示出优异的活性。此外，CMIEz 可重复使用超过 30 次而不降低效率。CMIEz 的实时应用通过水解酸预处理的玉米芯进行了验证。高效液相色谱显示，水解玉米芯中含有纤维二糖、葡萄糖、半乳糖、木糖、甘露糖和阿拉伯糖。结果表明，碳水化合物纳米颗粒可有效地从发酵液中包埋酶。
