Department of Pharmaceutical Biotechnology, Faculty of Pharmacy & Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, 1417614411, Iran.
Pharmaceutical Sciences Research Center, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
Biodegradation. 2022 Oct;33(5):489-508. doi: 10.1007/s10532-022-09994-x. Epub 2022 Jul 9.
An efficient heterogeneous natural polymer-based biocatalyst was fabricated through the immobilization of laccase onto dialdehyde inulin (DAI)-coated silica-caped magnetic nanoparticles (laccase@DAI@SiO@FeO⋅MNPs). The carrier was developed using SiO@FeO⋅MNPs and functionalized with DAI. The construction of immobilized laccase was confirmed by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy. Immobilization yield and efficiency were calculated as 61.0 ± 0.3% and 93.0 ± 0.6%, respectively. The immobilized laccase maintained 50% and 85% of its relative activity after 25 repeated cycles and 20 days of storage at 4 °C, respectively. The prepared biocatalyst effectively eliminated ofloxacin, a fluoroquinolone-type antibiotic, with a 63% removal capacity. Besides, antimicrobial activity study on some soil microorganisms involved in the biodegradation of xenobiotics revealed that the laccase-treated ofloxacin resulted in less toxic metabolites. The obtained data indicated that the fabricated biocatalyst is promising for the removal of ofloxacin or other analogs of fluoroquinolones in the environment.
通过将漆酶固定在醛基化菊粉（DAI）修饰的二氧化硅包裹的磁性纳米颗粒（laccase@DAI@SiO@FeO·MNPs）上，制备了一种高效的异质天然聚合物基生物催化剂。载体采用 SiO@FeO·MNPs 制备，并通过 DAI 进行功能化。通过扫描电子显微镜（SEM）和傅里叶变换红外（FTIR）光谱证实了固定化漆酶的构建。固定化漆酶的固定化产率和效率分别计算为 61.0±0.3%和 93.0±0.6%。固定化漆酶在 4°C 下储存 20 天后，经过 25 次重复循环，其相对活性仍保持在 50%和 85%。制备的生物催化剂能有效去除氟喹诺酮类抗生素氧氟沙星，去除率达 63%。此外，对一些参与异生物质生物降解的土壤微生物的抗菌活性研究表明，漆酶处理的氧氟沙星产生的代谢物毒性较小。所得数据表明，所制备的生物催化剂有望用于去除环境中的氧氟沙星或其他氟喹诺酮类抗生素。
