Department of Plastic Surgery, The Second Hospital of Anhui Medical University, Hefei, Anhui, China (mainland).
Med Sci Monit. 2020 May 28;26:e923808. doi: 10.12659/MSM.923808.
BACKGROUND Growing evidence indicates an association between microfibril-associated protein 2 (MFAP2) and a number of physiological and pathological mechanisms. The potential role of MFAP2 in cancer requires further elucidation. The present study investigated the biological behavior of MFAP2 in melanoma patients. MATERIAL AND METHODS MFAP2 inhibition was established in the B16 melanoma cell line through the use of RNA interference and was assessed by quantitative real-time PCR (qRT-PCR) and Western blot analysis. Wound-healing analysis, transwell assay, and in vivo imaging were performed to investigate the roles of MFAP2 reducing cell mobility, migration, and invasion abilities in vitro and in vivo. RESULTS We found substantially higher MFAP2 expression in B16 melanoma cells. The knockdown of MFAP2 inhibited B16 melanoma cells migration and invasion. Western blot analysis was used to assess changes in biomarkers of EMT, indicating the function of MFAP2 in EMT. We found that downregulation of MFAP2 altered the expression of Wnt/ß-catenin-linked protein. CONCLUSIONS Our results suggest that MFAP2 has potential as a molecular target to treat melanoma and suppress metastasis of melanoma cells.
越来越多的证据表明微纤维相关蛋白 2（MFAP2）与许多生理和病理机制有关。MFAP2 在癌症中的潜在作用需要进一步阐明。本研究探讨了 MFAP2 在黑色素瘤患者中的生物学行为。
通过使用 RNA 干扰，在 B16 黑色素瘤细胞系中建立 MFAP2 抑制，并通过定量实时 PCR（qRT-PCR）和 Western blot 分析进行评估。通过划痕愈合分析、Transwell 分析和体内成像来研究 MFAP2 降低细胞体外和体内迁移、侵袭能力的作用。
我们发现 B16 黑色素瘤细胞中 MFAP2 的表达明显升高。MFAP2 的敲低抑制了 B16 黑色素瘤细胞的迁移和侵袭。Western blot 分析用于评估 EMT 相关生物标志物的变化，表明 MFAP2 在 EMT 中的功能。我们发现下调 MFAP2 改变了 Wnt/β-连环蛋白相关蛋白的表达。
我们的结果表明，MFAP2 可能成为治疗黑色素瘤和抑制黑色素瘤细胞转移的分子靶点。
