从cDNA核苷酸序列推导的鲤鱼促性腺激素亚基的一级结构。

Primary structures of carp gonadotropin subunits deduced from cDNA nucleotide sequences.

作者信息

Chang Y S, Huang C J, Huang F L, Lo T B

机构信息

Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.

出版信息

Int J Pept Protein Res. 1988 Dec;32(6):556-64. doi: 10.1111/j.1399-3011.1988.tb01387.x.

DOI:10.1111/j.1399-3011.1988.tb01387.x

PMID:3246480

Abstract

The alpha and beta subunits of carp gonadotropin (cGTH) were isolated by high performance liquid chromatography. They were identified to be the subunits of cGTH by bioassay and by partial N-terminal amino acid sequence analysis. To elucidate the complete primary structures of the alpha and beta subunits of cGTH, cDNA cloning technique was employed. The alpha and beta subunits consist of 95 and 115 amino acid residues, respectively. Homology of the alpha subunit of cGTH to those of mammalian GTH is around 70%. In comparison, the extent of homology of the beta subunit between carp and salmon GTH (75%) is higher than that between fish and mammalian GTH (39-47%). Such comparative data suggest that the alpha subunit is highly conserved while the beta subunit is diversified during the molecular evolution of vertebrate GTH.

摘要

鲤鱼促性腺激素（cGTH）的α和β亚基通过高效液相色谱法分离。通过生物测定和部分N端氨基酸序列分析确定它们为cGTH的亚基。为了阐明cGTH的α和β亚基的完整一级结构，采用了cDNA克隆技术。α和β亚基分别由95和115个氨基酸残基组成。cGTH的α亚基与哺乳动物促性腺激素的α亚基的同源性约为70%。相比之下，鲤鱼和鲑鱼促性腺激素β亚基之间的同源性程度（75%）高于鱼类和哺乳动物促性腺激素之间的同源性程度（39-47%）。这些比较数据表明，在脊椎动物促性腺激素的分子进化过程中，α亚基高度保守，而β亚基则具有多样性。