Sugar A M, Picard M
Evans Memorial Department of Clinical Research, Boston University Medical Center, Massachusetts.
J Med Vet Mycol. 1988;26(6):321-6. doi: 10.1080/02681218880000461.
We describe a murine model of acute blastomycosis pneumonia induced by Blastomyces dermatitidis conidia. Anesthetized mice were infected by placing a droplet containing conidia suspended in sterile saline on the nares and allowing the suspension to be aspirated into the lungs. Conidia were obtained from mycelia derived from the yeast forms of two well characterized strains of B. dermatitidis, ATCC 26199 and 26197. ATCC 26199 yeast and conidia were highly virulent in BALB/cByJ mice whereas both forms of ATCC 26197 were incapable of causing disease in 60 days. Conidia and yeasts of the virulent strain caused pathologic changes primarily in the lungs, whereas no abnormalities were seen in the lungs, or elsewhere, in mice infected with the a virulent strain. Small numbers of ATCC 26199 yeast forms were found in scattered foci in liver and kidneys, but no inflammatory reaction surrounded the fungus. This murine model of acute blastomycosis pneumonia mimics the acute disease in humans and is initiated with the naturally infectious particle.
我们描述了一种由皮炎芽生菌分生孢子诱导的急性芽生菌病肺炎小鼠模型。将麻醉的小鼠通过在鼻孔放置一滴含有悬浮在无菌盐水中的分生孢子的悬液，并让悬液被吸入肺部来进行感染。分生孢子取自源自皮炎芽生菌两个特征明确的菌株ATCC 26199和26197酵母形式的菌丝体。ATCC 26199酵母和分生孢子在BALB/cByJ小鼠中具有高毒力，而ATCC 26197的两种形式在60天内都不能引起疾病。有毒力菌株的分生孢子和酵母主要在肺部引起病理变化，而在感染无毒力菌株的小鼠的肺部或其他部位未观察到异常。在肝脏和肾脏的散在病灶中发现少量ATCC 26199酵母形式，但真菌周围没有炎症反应。这种急性芽生菌病肺炎小鼠模型模拟了人类的急性疾病，并由天然感染性颗粒引发。
