Pereira Ana Paula Aparecida, Lauretti Leonardo Borges Chatagnier, Alvarenga Verônica Ortiz, Paulino Bruno Nicolau, Angolini Célio Fernando Figueiredo, Neri-Numa Iramaia Angelica, Orlando Eduardo Adilson, Pallone Juliana Azevedo Lima, Sant'Ana Anderson S, Pastore Glaucia Maria
Department of Food Science, Faculty of Food Engineering, University of Campinas (UNICAMP), Rua Monteiro Lobato, 80, CEP 13083-862 Campinas, São Paulo, Brazil.
Department of Food Science, Faculty of Food Engineering, University of Campinas (UNICAMP), Rua Monteiro Lobato, 80, CEP 13083-862 Campinas, São Paulo, Brazil.
Food Res Int. 2020 Jul;133:109187. doi: 10.1016/j.foodres.2020.109187. Epub 2020 Mar 20.
Fruta-do-lobo (Solanum lycocarpum St. Hill) is a native fruit commonly used in Brazilian folk medicine as a hypoglycemic agent. These properties are attributed to their starch, mainly its resistant fraction. Resistant starch has shown to increases the growth of Bifidobacterium and Lactobacillus in the gut, even though not being selective for these strains. In this scenario, this study aimed to investigate the potential prebiotic activity of fruta-do-lobo starch (FLS). FLS showed around 30% of resistant starch and their prebiotic potential was evaluated with five probiotic strains L. acidophilus (LA3 and LA5), L. casei (LC01) and B. animalis (BB12) and B. lactis (BLC1) in a concentration range of 1.0-2.0% of starch. In a preliminary screening, we evaluated, during 48 h, the viability of the starch with promoting growth agent. An increase in the growth of the probiotic strains tested was observed. We also evaluated the microorganism's metabolic activity by assessing the short-chain fatty acid (SCFA) production, using the best starch growth promotion conditions (2% of FLS and strains BLC1, LA5, and LC01). As expected, MRS and lactose were preferentially metabolized by BLC1, with the highest growth rates: 0.231 and 0.224 h, respectively. However, for this strain, the FLS growth rate (0.222 h) was 65% higher than FOS (0.144 h). Also, for LA5 FLS promoted higher growth (0.150 h) than FOS (0.135 h). Additionally, FLS promoted acetate production. These data are promising and indicate that FLS may have prebiotic potential and more studies need to be done with pathogenic microorganisms.
狼果（Solanum lycocarpum St. Hill）是一种本土水果，在巴西民间医学中常用作降血糖剂。这些特性归因于其淀粉，主要是其抗性部分。抗性淀粉已显示可促进肠道中双歧杆菌和乳酸杆菌的生长，尽管对这些菌株没有选择性。在这种情况下，本研究旨在调查狼果淀粉（FLS）的潜在益生元活性。FLS显示约30%的抗性淀粉，并在1.0 - 2.0%淀粉浓度范围内，用五种益生菌菌株嗜酸乳杆菌（LA3和LA5）、干酪乳杆菌（LC01）、动物双歧杆菌（BB12）和乳酸双歧杆菌（BLC1）评估其益生元潜力。在初步筛选中，我们在48小时内评估了添加促生长剂的淀粉的活力。观察到所测试的益生菌菌株的生长有所增加。我们还使用最佳淀粉生长促进条件（2%的FLS和菌株BLC1、LA5和LC01），通过评估短链脂肪酸（SCFA）的产生来评估微生物的代谢活性。正如预期的那样，BLC1优先代谢MRS和乳糖，生长速率最高，分别为0.231和0.224 h⁻¹。然而，对于该菌株，FLS的生长速率（0.222 h⁻¹）比低聚果糖（FOS，0.144 h⁻¹）高65%。此外，对于LA5，FLS促进的生长（0.150 h⁻¹）高于FOS（0.135 h⁻¹）。此外，FLS促进了乙酸盐的产生。这些数据很有前景，表明FLS可能具有益生元潜力，并且需要对致病微生物进行更多研究。
