• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Twist1 通过抑制三阴性乳腺癌中 Claudin15 的表达来加速肿瘤血管生成拟态。

Twist1 accelerates tumour vasculogenic mimicry by inhibiting Claudin15 expression in triple-negative breast cancer.

机构信息

Department of Pathology, Tianjin Medical University, Tianjin, China.

Department of Pathology, General Hospital of Tianjin Medical University, Tianjin, China.

出版信息

J Cell Mol Med. 2020 Jul;24(13):7163-7174. doi: 10.1111/jcmm.15167. Epub 2020 May 29.

DOI:10.1111/jcmm.15167
PMID:32469152
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7339217/
Abstract

The up-regulation of EMT regulator Twist1 has been implicated in vasculogenic mimicry (VM) formation in human triple-negative breast cancer (TNBC). Twist1 targets the Claudin15 promoter in hepatocellular carcinoma cells. Claudin family members are related with TNBC. However, the relationship between Claudin15 and VM formation is not clear. In this study, we first found that Claudin15 expression was frequently down-regulated in human TNBC, and Claudin15 down-regulation was significantly associated with VM and Twist1 nuclear expression. Claudin15 down-regulation correlated with shorter survival compared with high levels. Claudin15 silence significantly enhanced cell motility, invasiveness and VM formation in the non-TNBC MCF-7 cells. Conversely, an up-regulation of Claudin15 remarkably reduced TNBC MDA-MB-231 cell migration, invasion and VM formation. We also showed that down-regulation of Claudin15 was Twist1-dependent, and Twist1 repressed Claudin15 promoter activity. Furthermore, GeneChip analyses of mammary glands of Claudin15-deficient mice indicated that Claudin18 and Jun might be downstream factors of Twist1-Claudin15. Our results suggest that Twist1 induced VM through Claudin15 suppression in TNBC, and Twist1 inhibition of Claudin15 might involve Claudin18 and Jun expression.

摘要

EMT 调节因子 Twist1 的上调与人类三阴性乳腺癌（TNBC）中的血管生成拟态（VM）形成有关。Twist1 靶向肝癌细胞中的 Claudin15 启动子。Claudin 家族成员与 TNBC 有关。然而，Claudin15 与 VM 形成之间的关系尚不清楚。在这项研究中，我们首先发现 Claudin15 在人类 TNBC 中表达经常下调，Claudin15 的下调与 VM 和 Twist1 核表达显著相关。Claudin15 的下调与较短的生存期相关，与高水平相比。Claudin15 沉默显着增强了非 TNBC MCF-7 细胞的细胞迁移、侵袭和 VM 形成。相反，Claudin15 的上调显着降低了 TNBC MDA-MB-231 细胞的迁移、侵袭和 VM 形成。我们还表明，Claudin15 的下调是 Twist1 依赖性的，并且 Twist1 抑制 Claudin15 启动子活性。此外，Claudin15 缺陷小鼠乳腺的 GeneChip 分析表明，Claudin18 和 Jun 可能是 Twist1-Claudin15 的下游因子。我们的研究结果表明，Twist1 通过 TNBC 中 Claudin15 的抑制诱导 VM，而 Twist1 抑制 Claudin15 可能涉及 Claudin18 和 Jun 的表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889d/7339217/a6f97bc3913e/JCMM-24-7163-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889d/7339217/e9f678686b4c/JCMM-24-7163-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889d/7339217/35b75927cfac/JCMM-24-7163-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889d/7339217/ef86d7e30b77/JCMM-24-7163-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889d/7339217/0b6ab7adcc5d/JCMM-24-7163-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889d/7339217/a6f97bc3913e/JCMM-24-7163-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889d/7339217/e9f678686b4c/JCMM-24-7163-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889d/7339217/35b75927cfac/JCMM-24-7163-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889d/7339217/ef86d7e30b77/JCMM-24-7163-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889d/7339217/0b6ab7adcc5d/JCMM-24-7163-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/889d/7339217/a6f97bc3913e/JCMM-24-7163-g006.jpg

相似文献

1
Twist1 accelerates tumour vasculogenic mimicry by inhibiting Claudin15 expression in triple-negative breast cancer.Twist1 通过抑制三阴性乳腺癌中 Claudin15 的表达来加速肿瘤血管生成拟态。
J Cell Mol Med. 2020 Jul;24(13):7163-7174. doi: 10.1111/jcmm.15167. Epub 2020 May 29.
2
Twist1 expression induced by sunitinib accelerates tumor cell vasculogenic mimicry by increasing the population of CD133+ cells in triple-negative breast cancer.舒尼替尼诱导的Twist1表达通过增加三阴性乳腺癌中CD133+细胞的数量加速肿瘤细胞的血管生成拟态。
Mol Cancer. 2014 Sep 8;13:207. doi: 10.1186/1476-4598-13-207.
3
Anti-angiogenic treatment promotes triple-negative breast cancer invasion via vasculogenic mimicry.抗血管生成治疗通过血管生成拟态促进三阴性乳腺癌侵袭。
Cancer Biol Ther. 2017 Apr 3;18(4):205-213. doi: 10.1080/15384047.2017.1294288. Epub 2017 Feb 21.
4
Knockdown of long non-coding RNA TP73-AS1 suppresses triple negative breast cancer cell vasculogenic mimicry by targeting miR-490-3p/TWIST1 axis.长链非编码 RNA TP73-AS1 的敲低通过靶向 miR-490-3p/TWIST1 轴抑制三阴性乳腺癌细胞血管生成拟态。
Biochem Biophys Res Commun. 2018 Oct 12;504(4):629-634. doi: 10.1016/j.bbrc.2018.08.122. Epub 2018 Sep 5.
5
HIF-2α promotes the formation of vasculogenic mimicry in pancreatic cancer by regulating the binding of Twist1 to the VE-cadherin promoter.低氧诱导因子-2α通过调节Twist1与血管内皮钙黏蛋白启动子的结合来促进胰腺癌中血管生成拟态的形成。
Oncotarget. 2017 Jul 18;8(29):47801-47815. doi: 10.18632/oncotarget.17999.
6
Metastasis-associated in colon cancer-1 promotes vasculogenic mimicry in gastric cancer by upregulating TWIST1/2.结肠癌转移相关蛋白1通过上调TWIST1/2促进胃癌的血管生成拟态。
Oncotarget. 2015 May 10;6(13):11492-506. doi: 10.18632/oncotarget.3416.
7
Expression and functional significance of Twist1 in hepatocellular carcinoma: its role in vasculogenic mimicry.Twist1 在肝细胞癌中的表达及功能意义：在血管生成拟态中的作用。
Hepatology. 2010 Feb;51(2):545-56. doi: 10.1002/hep.23311.
8
Polyphyllin I suppresses the formation of vasculogenic mimicry via Twist1/VE-cadherin pathway.重楼皂苷 I 通过 Twist1/VE-cadherin 通路抑制血管生成拟态的形成。
Cell Death Dis. 2018 Sep 5;9(9):906. doi: 10.1038/s41419-018-0902-5.
9
Triptonide effectively inhibits triple-negative breast cancer metastasis through concurrent degradation of Twist1 and Notch1 oncoproteins.雷公藤红素通过降解 Twist1 和 Notch1 癌蛋白有效抑制三阴性乳腺癌转移。
Breast Cancer Res. 2021 Dec 18;23(1):116. doi: 10.1186/s13058-021-01488-7.
10
Hypoxia induced epithelial-mesenchymal transition and vasculogenic mimicry formation by promoting Bcl-2/Twist1 cooperation.缺氧通过促进Bcl-2/Twist1协同作用诱导上皮-间质转化和血管生成拟态形成。
Exp Mol Pathol. 2015 Oct;99(2):383-91. doi: 10.1016/j.yexmp.2015.08.009. Epub 2015 Aug 28.

引用本文的文献

1
The Interaction Between Vasculogenic Mimicry and the Immune System: Mechanistic Insights and Dual Exploration in Cancer Therapy.血管生成拟态与免疫系统之间的相互作用：癌症治疗中的机制洞察与双重探索
Cell Prolif. 2025 Jun;58(6):e13814. doi: 10.1111/cpr.13814. Epub 2025 Jan 26.
2
Identification of new subtypes of breast cancer based on vasculogenic mimicry related genes and a new model for predicting the prognosis of breast cancer.基于血管生成拟态相关基因的乳腺癌新亚型鉴定及乳腺癌预后预测新模型
Heliyon. 2024 Aug 20;10(17):e36565. doi: 10.1016/j.heliyon.2024.e36565. eCollection 2024 Sep 15.
3
Expression patterns of claudins in cancer.

本文引用的文献

1
Cancer Lipid Metabolism Confers Antiangiogenic Drug Resistance.癌症脂代谢赋予抗血管生成药物耐药性。
Cell Metab. 2018 Jul 3;28(1):104-117.e5. doi: 10.1016/j.cmet.2018.05.005. Epub 2018 May 31.
2
Dual roles of endothelial FGF-2-FGFR1-PDGF-BB and perivascular FGF-2-FGFR2-PDGFRβ signaling pathways in tumor vascular remodeling.内皮细胞FGF-2-FGFR1-PDGF-BB信号通路和血管周围FGF-2-FGFR2-PDGFRβ信号通路在肿瘤血管重塑中的双重作用
Cell Discov. 2018 Jan 16;4:3. doi: 10.1038/s41421-017-0002-1. eCollection 2018.
3
Off-tumor targets compromise antiangiogenic drug sensitivity by inducing kidney erythropoietin production.
紧密连接蛋白在癌症中的表达模式。
Heliyon. 2023 Oct 29;9(11):e21338. doi: 10.1016/j.heliyon.2023.e21338. eCollection 2023 Nov.
4
New Immunohistochemical Markers for Pleural Mesothelioma Subtyping.用于胸膜间皮瘤亚型分型的新型免疫组织化学标志物
Diagnostics (Basel). 2023 Sep 14;13(18):2945. doi: 10.3390/diagnostics13182945.
5
The Invasion and Metastasis of Colon Adenocarcinoma (COAD) Induced by SALL4.SALL4 诱导的结肠腺癌（COAD）的侵袭和转移。
J Immunol Res. 2022 Jun 1;2022:9385820. doi: 10.1155/2022/9385820. eCollection 2022.
6
Triptonide effectively inhibits triple-negative breast cancer metastasis through concurrent degradation of Twist1 and Notch1 oncoproteins.雷公藤红素通过降解 Twist1 和 Notch1 癌蛋白有效抑制三阴性乳腺癌转移。
Breast Cancer Res. 2021 Dec 18;23(1):116. doi: 10.1186/s13058-021-01488-7.
7
Recent Advances in Glioma Therapy: Combining Vascular Normalization and Immune Checkpoint Blockade.神经胶质瘤治疗的最新进展：血管正常化与免疫检查点阻断相结合
Cancers (Basel). 2021 Jul 22;13(15):3686. doi: 10.3390/cancers13153686.
肿瘤外靶点通过诱导肾脏产生促红细胞生成素而降低抗血管生成药物的敏感性。
Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):E9635-E9644. doi: 10.1073/pnas.1703431114. Epub 2017 Oct 23.
4
Preclinical and clinical applications of specific molecular imaging for HER2-positive breast cancer.HER2阳性乳腺癌特异性分子成像的临床前和临床应用
Cancer Biol Med. 2017 Aug;14(3):271-280. doi: 10.20892/j.issn.2095-3941.2017.0044.
5
Maintenance of antiangiogenic and antitumor effects by orally active low-dose capecitabine for long-term cancer therapy.长期癌症治疗中口服低剂量卡培他滨维持抗血管生成和抗肿瘤作用。
Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):E5226-E5235. doi: 10.1073/pnas.1705066114. Epub 2017 Jun 12.
6
Discontinuation of anti-VEGF cancer therapy promotes metastasis through a liver revascularization mechanism.抗血管内皮生长因子癌症治疗的停药会通过肝脏再血管化机制促进转移。
Nat Commun. 2016 Sep 1;7:12680. doi: 10.1038/ncomms12680.
7
Lyn modulates Claudin-2 expression and is a therapeutic target for breast cancer liver metastasis.Lyn调节Claudin-2的表达，是乳腺癌肝转移的治疗靶点。
Oncotarget. 2015 Apr 20;6(11):9476-87. doi: 10.18632/oncotarget.3269.
8
ZEB2 promotes vasculogenic mimicry by TGF-β1 induced epithelial-to-mesenchymal transition in hepatocellular carcinoma.ZEB2通过TGF-β1诱导的上皮-间质转化促进肝细胞癌的血管生成拟态。
Exp Mol Pathol. 2015 Jun;98(3):352-9. doi: 10.1016/j.yexmp.2015.03.030. Epub 2015 Mar 26.
9
YAP1 Regulates OCT4 Activity and SOX2 Expression to Facilitate Self-Renewal and Vascular Mimicry of Stem-Like Cells.YAP1调节OCT4活性和SOX2表达以促进干细胞样细胞的自我更新和血管生成拟态。
Stem Cells. 2015 Jun;33(6):1705-18. doi: 10.1002/stem.1993.
10
Dual effects of collagenase-3 on melanoma: metastasis promotion and disruption of vasculogenic mimicry.胶原酶-3对黑色素瘤的双重作用：促进转移及破坏血管生成拟态
Oncotarget. 2015 Apr 20;6(11):8890-9. doi: 10.18632/oncotarget.3189.