School of Food Sciences and Engineering, South China University of Technology, Guangzhou 510641, China.
Department of Food Science, Stocking Hall, Cornell University, Ithaca, NY 14853, USA.
Food Funct. 2020 Jun 24;11(6):5409-5419. doi: 10.1039/d0fo00891e.
Increased consumption of fruits and vegetables is associated with reduced risk of age-related functional declines and chronic diseases, primarily attributed to their bioactive phytochemicals. Apples and blueberries are rich in phytochemicals with a wide range of biological activities and health benefits. Our previous research has shown the combination of apple peel extracts (APE) and blueberry extracts (BE) can synergistically promote the lifespan of Caenorhabditis elegans (C. elegans). The objectives of this study were to determine whether the extension of lifespan was involved in regulation of oxidative stress, and to explore the underlying mechanisms of action. The results showed that the combination of APE and BE could synergistically ameliorate oxidative stress by improving antioxidant enzyme activities and enhancing resistance to paraquat. Meanwhile, treatment with APE plus BE could down-regulate the overexpression of reactive oxygen species (ROS) and affect the expression of antioxidant related genes, including sod-3, cat-1, ctl-1, skn-1, mev-1 and isp-1. However, administration with APE plus BE abolished the extension of the lifespan of skn-1(zu135) mutants, and inhibited the expression of skn-1 downstream genes, including gcs-1, gst-4 and gst-7. In addition, supplementation with APE plus BE could promote the migration of SKN-1 into the nucleus, which eliminated improvement to ROS and paraquat. In conclusion, the combination of APE and BE could synergistically protect against oxidative stress in C. elegans via the SKN-1/Nrf2 pathway. This study provided the theoretical basis to explore the combination of phytochemicals in the prevention of aging regulated by oxidative stress.
增加水果和蔬菜的摄入量与降低与年龄相关的功能下降和慢性疾病的风险有关，这主要归因于它们的生物活性植物化学物质。苹果和蓝莓富含具有广泛生物活性和健康益处的植物化学物质。我们之前的研究表明，苹果皮提取物 (APE) 和蓝莓提取物 (BE) 的组合可以协同促进秀丽隐杆线虫 (C. elegans) 的寿命延长。本研究的目的是确定寿命的延长是否涉及氧化应激的调节，并探讨其作用机制。结果表明，APE 和 BE 的组合可以通过提高抗氧化酶活性和增强对百草枯的抗性来协同改善氧化应激。同时，APE 加 BE 处理可以下调活性氧 (ROS) 的过表达，并影响抗氧化相关基因的表达，包括 sod-3、cat-1、ctl-1、skn-1、mev-1 和 isp-1。然而，APE 加 BE 的给药消除了 skn-1(zu135)突变体寿命的延长，并抑制了 skn-1 下游基因的表达，包括 gcs-1、gst-4 和 gst-7。此外，APE 加 BE 的补充可以促进 SKN-1 进入细胞核的迁移，从而消除了对 ROS 和百草枯的改善。综上所述，APE 和 BE 的组合可以通过 SKN-1/Nrf2 途径协同保护秀丽隐杆线虫免受氧化应激。这项研究为探索植物化学物质在预防由氧化应激调节的衰老方面的组合提供了理论依据。
