基于核磁共振代谢组学的三疣梭子蟹金属污染及其生物效应。
Metal pollution and its biological effects in swimming crab Portunus trituberculatus by NMR-based metabolomics.
机构信息
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, PR China.
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, PR China.
出版信息
Mar Pollut Bull. 2020 Aug;157:111307. doi: 10.1016/j.marpolbul.2020.111307. Epub 2020 May 25.
Metal pollution in the Bohai Sea in China has posed a potential risk on marine organisms. In this work, crabs (Portunus trituberculatus) were sampled from four sites, namely a reference (site 3934) and three metal-polluted (sites 6151, 6351, and 3562) sites, located in the Bohai Sea. Metal concentrations in crab gill tissues were measured using inductively coupled plasma mass spectrometry. Cu, Zn, and Cd in crab samples from S3562 presented the highest concentrations. Particularly, Cu concentration exceeded the marine biological quality standard II. Cd contents in crab samples from all metal-polluted sites exceeded the marine biological quality standard I. Nuclear magnetic resonance-based metabolomics indicated metal pollution-induced immune stresses in crab samples from all metal-polluted sites. Metal pollution in S6151 and S6351 disturbed energy metabolism through differential pathways. For crab samples from S3562, the metabolic profile suggested that metal pollution mainly induced osmotic stress.
中国渤海的金属污染对海洋生物构成了潜在风险。在这项工作中，从四个地点（一个参照点[3934 号地点]和三个金属污染点[6151 号、6351 号和 3562 号地点]）采集了螃蟹（三疣梭子蟹）样本，这些地点位于渤海。采用电感耦合等离子体质谱法测量了螃蟹鳃组织中的金属浓度。来自 S3562 的螃蟹样本中的 Cu、Zn 和 Cd 浓度最高。特别是，Cu 浓度超过了海洋生物质量标准 II。所有金属污染点的螃蟹样本中的 Cd 含量均超过了海洋生物质量标准 I。基于核磁共振的代谢组学表明，所有金属污染点的螃蟹样本都受到了金属污染引起的免疫应激。S6151 和 S6351 中的金属污染通过不同的途径干扰了能量代谢。对于来自 S3562 的螃蟹样本，代谢谱表明金属污染主要诱导了渗透胁迫。
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