Wang Shuang, Ji Chenglong, Li Fei, Wu Huifeng
CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai 264003, P. R. China; University of Chinese Academy of Sciences, Beijing 100049, P. R. China.
CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai 264003, P. R. China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, P. R. China; Center for Ocean Mega-Science, Chinese Academy of Sciences (CAS), Qingdao 266071, P. R. China.
Ecotoxicol Environ Saf. 2022 Apr 1;234:113416. doi: 10.1016/j.ecoenv.2022.113416. Epub 2022 Mar 14.
Cadmium (Cd) is one of the typical metal pollutants in the Bohai Sea. To evaluate the acute toxicological effects of Cd on marine crustaceans, juvenile Fenneropenaeus chinensis and Portunus trituberculatus were exposed to Cd at environmentally relevant concentrations (5 and 50 μg/L) for 96 h. Cd accumulation, antioxidants and metabolite profiles were characterized to elucidate the responses of juvenile crustaceans to Cd stress. Significant Cd accumulation was observed in both juvenile crustaceans in 50 μg/L Cd-treated group. Results showed that Cd exposure induced hormesis based on the alterations of GSH, SOD and CAT activities (i.e. increased levels in the low concentration of Cd treatment and recovered levels in the high concentration of Cd treatment) in juvenile P. trituberculatus. Similarly, the responses of GSH contents presented hormesis pattern in Cd-treated juvenile F. chinensis. Na-K-ATPase contents were significantly elevated in 50 μg/L Cd-treated group. In addition, untargeted NMR-based metabolomics indicated Cd caused the disturbance in osmotic regulation and energy consumption in both juvenile F. chinensis and P. trituberculatus via different pathways. The immunotoxicity and movement disorder were uniquely demonstrated in juvenile P. trituberculatus after Cd exposure.
镉(Cd)是渤海典型的金属污染物之一。为评估镉对海洋甲壳类动物的急性毒理学效应,将中华哲水蚤幼体和三疣梭子蟹幼体暴露于环境相关浓度(5和50μg/L)的镉中96小时。对镉积累、抗氧化剂和代谢物谱进行表征,以阐明幼体甲壳类动物对镉胁迫的反应。在50μg/L镉处理组的两种幼体甲壳类动物中均观察到显著的镉积累。结果表明,基于三疣梭子蟹幼体中谷胱甘肽(GSH)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性的变化(即低浓度镉处理时水平升高,高浓度镉处理时恢复到正常水平),镉暴露诱导了兴奋效应。同样,在镉处理的中华哲水蚤幼体中,GSH含量的反应呈现出兴奋效应模式。在50μg/L镉处理组中,钠钾ATP酶含量显著升高。此外,基于非靶向核磁共振的代谢组学表明,镉通过不同途径导致中华哲水蚤幼体和三疣梭子蟹幼体的渗透调节和能量消耗紊乱。镉暴露后,三疣梭子蟹幼体独特地表现出免疫毒性和运动障碍。