Rojas Hanen Fernandez, Lima Amorce, Kubasek Carly, Gostnell Alicia, Silbert Suzane
Esoteric Testing/R&D Laboratory, Tampa General Hospital, 1 Tampa General Cir, Tampa, FL, 33606, USA.
Esoteric Testing/R&D Laboratory, Tampa General Hospital, 1 Tampa General Cir, Tampa, FL, 33606, USA..
Diagn Microbiol Infect Dis. 2020 Aug;97(4):115055. doi: 10.1016/j.diagmicrobio.2020.115055. Epub 2020 Apr 6.
The objectives of this study were to assess the ideal volume of Copan FecalSwab™ (FS) preserved stool sample to use with the BD MAX™ Enteric Bacterial Panel and the Extended Enteric Bacterial Panel (BDM GIP) and to compare the performance of FS to the recommended Meridian Para-Pak Cary-Blair medium (PP) for the BDM GIP. Three different input volumes (10, 25, and 50 μL) of stool inoculated with American Type Culture Collection strains representing the targets detected by BDM GIP were tested. Additionally, 144 unpreserved stool samples submitted for gastrointestinal (GI) testing were transferred to PP and FS media and tested by the BDM GIP using 10 μL of PP and 50 μL of FS media. A 100% agreement was observed between PP and FS results. The performance of 50 μL of FS stool preserved sample was equivalent to 10 μL of traditional Cary-Blair PP preserved specimens for GI pathogens detection using the BDM GIP.
本研究的目的是评估用于BD MAX™肠道细菌检测板和扩展肠道细菌检测板（BDM GIP）的Copan粪便拭子™（FS）保存粪便样本的理想体积，并将FS与推荐用于BDM GIP的Meridian Para-Pak Cary-Blair培养基（PP）的性能进行比较。测试了接种有代表BDM GIP检测目标的美国典型培养物保藏中心菌株的三种不同输入体积（10、25和50μL）的粪便。此外，将144份提交用于胃肠道（GI）检测的未保存粪便样本转移至PP和FS培养基中，并使用10μL的PP和50μL的FS培养基通过BDM GIP进行检测。PP和FS结果之间观察到100%的一致性。使用BDM GIP检测胃肠道病原体时，50μL的FS粪便保存样本的性能等同于10μL传统Cary-Blair PP保存标本的性能。
