Goneau Lee W, Mazzulli Anthony, Trimi Xhermi, Cabrera Ana, Lo Pauline, Mazzulli Tony
Public Health Ontario, 661 University Avenue, Toronto, Ontario, M5G 1M1, Canada.
Department of Microbiology, University Health Network/Sinai Health System, Toronto, Ontario, M5G 1X5, Canada.
Diagn Microbiol Infect Dis. 2019 May;94(1):15-21. doi: 10.1016/j.diagmicrobio.2018.11.020. Epub 2018 Dec 11.
The isolation of stool pathogens is difficult due to their fastidious nature and the rapid overgrowth of fecal flora. In this study, we evaluate the preservation and isolation of enteric pathogens from stool using the automated COPAN Walk-Away Specimen Processor (WASP®) in conjunction with FecalSwab™ and selenite media. Pathogen viability and fecal commensal abundance were stable in FecalSwab™ media under both room-temperature and refrigerated incubation conditions, resulting in a significantly increased number of well-isolated pathogen colonies observed when compared to samples incubated in modified Cary-Blair media. Isolation of individual pathogen colonies was improved via WASP® planting compared to those planted using the Isoplater system. Furthermore, preincubation using the newly formulated COPAN selenite media significantly enhanced the yield of Salmonella enterica serovar Typhimurium. Together, the automated WASP® system combined with FecalSwab™ and selenite media represents a rapid and efficient approach for the processing of stool specimens compared to standard methods.
由于粪便病原体的苛求特性以及粪便菌群的快速过度生长，分离粪便病原体具有一定难度。在本研究中，我们评估了使用自动COPAN Walk-Away标本处理仪（WASP®）结合FecalSwab™和亚硒酸盐培养基从粪便中保存和分离肠道病原体的情况。在室温及冷藏培养条件下，病原体活力和粪便共生菌丰度在FecalSwab™培养基中均保持稳定，与在改良Cary-Blair培养基中培养的样本相比，观察到的分离良好的病原体菌落数量显著增加。与使用Isoplater系统接种的菌落相比，通过WASP®接种可提高单个病原体菌落的分离效果。此外，使用新配制的COPAN亚硒酸盐培养基进行预培养可显著提高肠炎沙门氏菌鼠伤寒血清型的分离率。与标准方法相比，自动WASP®系统结合FecalSwab™和亚硒酸盐培养基代表了一种快速有效的粪便标本处理方法。
