A Highly Selective and Sensitive Chemiluminescent Probe for Real-Time Monitoring of Hydrogen Peroxide in Cells and Animals.

Selective and sensitive molecular probes for hydrogen peroxide (H O ), which plays diverse roles in oxidative stress and redox signaling, are urgently needed to investigate the physiological and pathological effects of H O . A lack of reliable tools for in vivo imaging has hampered the development of H O mediated therapeutics. By combining a specific tandem Payne/Dakin reaction with a chemiluminescent scaffold, H O -CL-510 was developed as a highly selective and sensitive probe for detection of H O both in vitro and in vivo. A rapid 430-fold enhancement of chemiluminescence was triggered directly by H O without any laser excitation. Arsenic trioxide induced oxidative damage in leukemia was successfully detected. In particular, cerebral ischemia-reperfusion injury-induced H O fluxes were visualized in rat brains using H O -CL-510, providing a new chemical tool for real-time monitoring of H O dynamics in living animals.