Morningside Laboratory for Chemical Biology and Department of Chemistry, The University of Hong Kong Shenzhen Institute of Research and Innovation (HKU-SIRI), The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China.
School of Chemistry, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, 69978, Israel.
Angew Chem Int Ed Engl. 2020 Aug 17;59(34):14326-14330. doi: 10.1002/anie.202005429. Epub 2020 Jul 1.
Selective and sensitive molecular probes for hydrogen peroxide (H O ), which plays diverse roles in oxidative stress and redox signaling, are urgently needed to investigate the physiological and pathological effects of H O . A lack of reliable tools for in vivo imaging has hampered the development of H O mediated therapeutics. By combining a specific tandem Payne/Dakin reaction with a chemiluminescent scaffold, H O -CL-510 was developed as a highly selective and sensitive probe for detection of H O both in vitro and in vivo. A rapid 430-fold enhancement of chemiluminescence was triggered directly by H O without any laser excitation. Arsenic trioxide induced oxidative damage in leukemia was successfully detected. In particular, cerebral ischemia-reperfusion injury-induced H O fluxes were visualized in rat brains using H O -CL-510, providing a new chemical tool for real-time monitoring of H O dynamics in living animals.
用于检测过氧化氢（H2O2）的选择性和高灵敏度分子探针在氧化应激和氧化还原信号转导中发挥着多种作用，因此人们急需开发这类探针以研究 H2O2 的生理和病理效应。由于缺乏可靠的体内成像工具，H2O2 介导的治疗方法的发展受到了阻碍。本研究通过将特异性串联 Payne/Dakin 反应与化学发光支架相结合，开发出 H2O2-CL-510 作为一种高选择性和高灵敏度的探针，可用于体外和体内检测 H2O2。H2O2 可直接触发化学发光增强 430 倍，无需激光激发。三氧化二砷诱导的白血病氧化损伤得到了成功检测。特别是，使用 H2O2-CL-510 可视化了大鼠脑缺血再灌注损伤诱导的 H2O2 流，为实时监测活体动物中 H2O2 动力学提供了新的化学工具。
