Southern University and A and M College, Baton Rouge, LA 70813,
sddsf.
Front Biosci (Landmark Ed). 2020 Jun 1;25(10):1894-1900. doi: 10.2741/4883.
We analyzed the nucleocapsid and surface proteins from several Coronaviridae viruses using an alignment-free computer program. Three isolates of novel, human coronavirus (SARS0CoV-2) (2019) that are responsible for the current pandemic and older SARS strains of human and animal coronaviruses were examined. The nucleocapsid and glycoprotein sequences are identical for the three novel 2019 human isolates and they are closely related to these sequences in six bat and human SARS coronaviruses. This strongly supports the bat origin of the pandemic, novel coronavirus. One surface glycoprotein fragment of 111 amino acids is the largest, conserved, common permutation in the examined bat SARS-like and human SARS viruses, including the Covid-19 virus. BLAST analysis confirmed that this fragment is conserved only in the human and bat SARS strains. This fragment likely is involved in infectivity and is of interest for vaccine development. Surface glycoprotein and nucleocapsid protein sequence homologies of 58.9% and 82.5%, respectively, between the novel SARS0CoV-2 strains and the human SARS (2018) virus suggest that existing anti-SARS vaccines may provide some protection against the novel coronavirus.
我们使用一种无需序列比对的计算机程序分析了几种冠状病毒的核衣壳和表面蛋白。我们检测了三种新型人冠状病毒（SARS-CoV-2）（2019 年），这些病毒是当前大流行的罪魁祸首，还有人和动物冠状病毒的旧 SARS 株。三种新型 2019 年人分离株的核衣壳和糖蛋白序列相同，与六种蝙蝠和人类 SARS 冠状病毒的这些序列密切相关。这强烈支持了大流行新型冠状病毒源自蝙蝠。在检测到的蝙蝠 SARS 样和人类 SARS 病毒中，包括新冠病毒在内，有一个 111 个氨基酸的表面糖蛋白片段是最大、最保守、最常见的排列。BLAST 分析证实，该片段仅在人和蝙蝠 SARS 株中保守。该片段可能与感染性有关，是疫苗开发的关注点。新型 SARS-CoV-2 株与人类 SARS（2018）病毒之间的表面糖蛋白和核衣壳蛋白序列同源性分别为 58.9%和 82.5%，这表明现有的抗 SARS 疫苗可能对新型冠状病毒提供一定的保护。
