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Enhancing in planta gene targeting efficiencies in Arabidopsis using temperature-tolerant CRISPR/LbCas12a.

作者信息

Merker Laura, Schindele Patrick, Huang Teng-Kuei, Wolter Felix, Puchta Holger

机构信息

Botanical Institute, Karlsruhe Institute of Technology, Karlsruhe, Germany.

出版信息

Plant Biotechnol J. 2020 Dec;18(12):2382-2384. doi: 10.1111/pbi.13426. Epub 2020 Aug 18.

DOI:10.1111/pbi.13426
PMID:32473055
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7680533/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8495/11386667/7eed8e4ca2cf/PBI-18-2382-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8495/11386667/7eed8e4ca2cf/PBI-18-2382-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8495/11386667/7eed8e4ca2cf/PBI-18-2382-g001.jpg

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Plant Biotechnol J. 2020 Oct;18(10):2133-2143. doi: 10.1111/pbi.13373. Epub 2020 Apr 1.
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CRISPR-Cas12a enables efficient biallelic gene targeting in rice.CRISPR-Cas12a实现了水稻中高效的双等位基因靶向。
Plant Biotechnol J. 2020 Jun;18(6):1351-1353. doi: 10.1111/pbi.13295. Epub 2019 Nov 27.
3
Engineering CRISPR/LbCas12a for highly efficient, temperature-tolerant plant gene editing.
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Sci Rep. 2024 Dec 28;14(1):30791. doi: 10.1038/s41598-024-80352-y.
4
Crop genome editing through tissue-culture-independent transformation methods.通过不依赖组织培养的转化方法进行作物基因组编辑。
Front Genome Ed. 2024 Dec 5;6:1490295. doi: 10.3389/fgeed.2024.1490295. eCollection 2024.
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BMC Plant Biol. 2024 Jul 12;24(1):665. doi: 10.1186/s12870-024-05375-z.
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Enhanced editing efficiency in Arabidopsis with a LbCas12a variant harboring D156R and E795L mutations.携带D156R和E795L突变的LbCas12a变体提高了拟南芥中的编辑效率。
aBIOTECH. 2024 Mar 26;5(2):117-126. doi: 10.1007/s42994-024-00144-w. eCollection 2024 Jun.
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