Streeter A J, Hoener B A
Department of Pharmacy, School of Pharmacy, University of California, San Francisco 94143.
Pharm Res. 1988 Jul;5(7):434-6. doi: 10.1023/a:1015988401601.
We have shown that the xanthine oxidase-catalyzed anaerobic reduction of nitrofurazone in the presence of added DNA leads to the formation of covalently bound adducts. Further, by systematically decreasing the pH of the reaction mixture, we have demonstrated that generation of the reactive species is facilitated under mildly acidic conditions. From these observations, we conclude that it is the nitrenium ion formed from nitrofurazone which binds to DNA.
我们已经表明，在添加DNA的情况下，黄嘌呤氧化酶催化的呋喃西林厌氧还原会导致共价结合加合物的形成。此外，通过系统地降低反应混合物的pH值，我们证明了在轻度酸性条件下会促进活性物种的生成。基于这些观察结果，我们得出结论，与DNA结合的是由呋喃西林形成的氮宾离子。
