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Improved sensitivity using a dual target, E and RdRp assay for the diagnosis of SARS-CoV-2 infection: Experience at a large NHS Foundation Trust in the UK.

作者信息

Colton Hayley, Ankcorn Michael, Yavuz Mehmet, Tovey Leeanne, Cope Alison, Raza Mohammad, Keeley Alexander J, State Amy, Poller Bozena, Parker Matthew, de Silva Thushan I, Evans Cariad

机构信息

Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, United Kingdom.

Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield, United Kingdom.

出版信息

J Infect. 2021 Jan;82(1):159-198. doi: 10.1016/j.jinf.2020.05.061. Epub 2020 May 28.

DOI:10.1016/j.jinf.2020.05.061
PMID:32474037
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7255707/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9456/7255707/de5d56ab375d/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9456/7255707/de5d56ab375d/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9456/7255707/de5d56ab375d/gr1_lrg.jpg

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J Infect. 2020 Jun;80(6):671-693. doi: 10.1016/j.jinf.2020.02.008. Epub 2020 Feb 22.
3
Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.实时 RT-PCR 检测 2019 新型冠状病毒(2019-nCoV)
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J Arthropod Borne Dis. 2021 Dec 31;15(4):358-365. doi: 10.18502/jad.v15i4.10500. eCollection 2021 Dec.
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Altered subgenomic RNA abundance provides unique insight into SARS-CoV-2 B.1.1.7/Alpha variant infections.亚基因组 RNA 丰度的改变为深入了解 SARS-CoV-2 B.1.1.7/Alpha 变体感染提供了独特的视角。
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