Department of Experimental Allergology and Immunology, Medical University of Bialystok, Bialystok, Poland; Department of Allergology and Internal Medicine, Medical University of Bialystok, Poland.
Department of Allergology and Internal Medicine, Medical University of Bialystok, Poland.
Adv Med Sci. 2020 Sep;65(2):304-309. doi: 10.1016/j.advms.2020.05.003. Epub 2020 May 28.
Recognition of individual allergens by IgE is crucial for triggering symptoms in allergic rhinitis (AR) or asthmatic (AA) patients. House dust mite (HDM) allergy is frequent around the world, the sensitization profile to individual HDM allergens varies in individual HDM-allergic patients (APs). The aim of this study was to evaluate the pattern of IgE sensitization to three major Dermatophagoides pteronyssinus (Dp) allergens among patients from North Eastern Poland suffering from HDM-AR and/or AA.
The study was performed on 323 HDM-AR and/or AA patients and 106 controls (CG) including 30 healthy non-atopic subjects, 32 AR patients not sensitized to Dp and 44 non-atopic asthmatics. IgE levels to natural (n)Der p 1, nDer p 2, recombinant (r)Der p 2.0101 and rDer p 23 allergens were measured by ELISA.
The majority of HDM-APs were sensitized to nDer p 1 (72.1%), nDer p 2 (81.7%), rDer p 2.0101 (78.3%) and rDer p 23 (70.9%). The frequency of positive results to individual allergens depended on clinical manifestations and the level of IgE to the whole Dp extract. In HDM-AA patients, reactivity to nDer p 1 and rDer p 23 was detected more frequently than in HDM-AR patients. The whole Dp extract completely inhibited IgE binding to nDer p 1 and nDer p 2 but only partially to rDer p 23.
HDM-APs from North-Eastern Poland display sensitization profile to major allergens which is similarly observed in western Europe. HDM-based diagnostic and therapeutic products should include all major allergens.
IgE 对个体过敏原的识别对于引发过敏性鼻炎（AR）或哮喘（AA）患者的症状至关重要。屋尘螨（HDM）过敏在世界各地很常见，个体 HDM 过敏患者（AP）对个体 HDM 过敏原的致敏谱也各不相同。本研究旨在评估来自波兰东北部的 HDM-AR 和/或 AA 患者对三种主要粉尘螨（Dp）过敏原的 IgE 致敏模式。
该研究共纳入 323 名 HDM-AR 和/或 AA 患者和 106 名对照者（CG），包括 30 名健康非特应性个体、32 名对 Dp 无致敏的 AR 患者和 44 名非特应性哮喘患者。通过 ELISA 法检测天然（n）Der p 1、nDer p 2、重组（r）Der p 2.0101 和 rDer p 23 过敏原的 IgE 水平。
大多数 HDM-AP 对 nDer p 1（72.1%）、nDer p 2（81.7%）、rDer p 2.0101（78.3%）和 rDer p 23（70.9%）过敏。个体过敏原的阳性结果频率取决于临床表现和整个 Dp 提取物的 IgE 水平。在 HDM-AA 患者中，对 nDer p 1 和 rDer p 23 的反应性比 HDM-AR 患者更常见。整个 Dp 提取物完全抑制 nDer p 1 和 nDer p 2 与 IgE 的结合，但仅部分抑制 rDer p 23 与 IgE 的结合。
来自波兰东北部的 HDM-AP 对主要过敏原的致敏谱与西欧相似。基于 HDM 的诊断和治疗产品应包含所有主要过敏原。
