Division of Pulmonary and Critical Care Medicine, Department of Medicine, National Jewish Health, Denver, CO, USA.
University of Colorado Cancer Center, Aurora, CO, USA.
Methods Mol Biol. 2020;2155:125-140. doi: 10.1007/978-1-0716-0655-1_11.
Tissue resident mesenchymal progenitor cells (MPC) are important regulators of tissue repair or regeneration, remodeling, inflammation, and angiogenesis. Here we describe a technology used to define, isolate, and characterize a population of resident lung MPC in both human and mouse explanted tissue. The definition of this population using a defined set of markers facilitates the repeatable isolation of a mesenchymal subpopulation population by flow cytometry and the subsequent translational study of this specific cell type and function.
组织驻留间充质祖细胞（MPC）是组织修复或再生、重塑、炎症和血管生成的重要调节因子。在这里，我们描述了一种用于定义、分离和表征人源和鼠源离体组织中驻留肺 MPC 群体的技术。使用定义的一组标记物定义该群体，有助于通过流式细胞术重复分离间充质亚群，并随后对该特定细胞类型和功能进行转化研究。
