Chow Kelsey S, Jun DuHyun, Helm Karen M, Wagner David H, Majka Susan M
Charles C. Gates Regenerative Medicine and Stem Cell Biology Program, University of Colorado Denver, USA.
J Vis Exp. 2011 Oct 26(56):e3159. doi: 10.3791/3159.
Tissue resident mesenchymal stem cells (MSC) are important regulators of tissue repair or regeneration, fibrosis, inflammation, angiogenesis and tumor formation. Taken together these studies suggest that resident lung MSC play a role during pulmonary tissue homeostasis, injury and repair during diseases such as pulmonary fibrosis (PF) and arterial hypertension (PAH). Here we describe a technology to define a population of resident lung MSC. The definition of this population in vivo pulmonary tissue using a define set of markers facilitates the repeated isolation of a well-characterized stem cell population by flow cytometry and the study of a specific cell type and function.
组织驻留间充质干细胞（MSC）是组织修复或再生、纤维化、炎症、血管生成和肿瘤形成的重要调节因子。综合这些研究表明，驻留肺MSC在诸如肺纤维化（PF）和动脉高血压（PAH）等疾病的肺组织稳态、损伤和修复过程中发挥作用。在此，我们描述一种定义驻留肺MSC群体的技术。使用一组定义的标志物在体内肺组织中定义该群体，有助于通过流式细胞术重复分离特征明确的干细胞群体，并研究特定细胞类型及其功能。
