IRDL UMR 6027, Université Bretagne Sud, 56000 Vannes, France.
Dipartimento di Scienze Biomediche, Odontoiatriche e delle ImmaginiMorfologiche e Funzionali. University of Messina, Italy.
Int J Environ Health Res. 2022 Feb;32(2):252-263. doi: 10.1080/09603123.2020.1749573. Epub 2020 May 30.
For several years, environmental exogenous agents, called endocrine disruptors, are suspected to interfere with the essential functions of reproduction and development in many living organisms. In this study, endocrine disruptors including five phthalates and two bisphenols contents in finished products were assayed and their estrogenic activity were measured by using the Yeast Estrogen Screen system with respect to human and trout estrogen receptors hERα and rtER. Independently of the estrogen receptor, only short-chain phthalates (DBP and BBP) and the two bisphenols exhibited an estrogenic activity. Besides, the risk of three end-products (agro-food, cosmetics, and pharmaceutical) was evaluated before and after forced aging. Only two cosmetics the face cream and the perfume presented a hazard which increases with aging. These results are consistent with the compounds identified by Gas chromatography-mass spectrometry. These findings confirmed that the YES system can be routinely used to evaluate the estrogenic hazards within finished products.
几年来，环境外源物质，称为内分泌干扰物，被怀疑会干扰许多生物的生殖和发育的基本功能。在这项研究中，测定了成品中包括五种邻苯二甲酸酯和两种双酚在内的内分泌干扰物的含量，并利用酵母雌激素筛选系统，用人和鳟鱼雌激素受体 hERα 和 rtER 测量了它们的雌激素活性。与雌激素受体无关，只有短链邻苯二甲酸酯（DBP 和 BBP）和两种双酚表现出雌激素活性。此外，还评估了三种终端产品（农业食品、化妆品和制药）在强制老化前后的风险。只有两种化妆品——面霜和香水——存在风险，而且随着老化风险增加。这些结果与气相色谱-质谱法鉴定的化合物一致。这些发现证实，YES 系统可常规用于评估成品中的雌激素危害。
