Reproductive Medicine Department, Jinan Central Hospital, Shandong First Medical University & Shandong Academy of Medical Sciences , Jinan, China.
Reproductive Medicine Department, Jinan Central Hospital Affiliated to Shandong University , Jinan, China.
Syst Biol Reprod Med. 2020 Oct;66(5):322-328. doi: 10.1080/19396368.2020.1764132. Epub 2020 May 31.
As the final and critical step in fertilization (IVF), embryo transfer has always received much attention and deserves continuous optimization. In the present study, to explore the role of autocrine factors in embryo self-spent culture media, we prospectively compared embryo transfer with self-spent culture medium and fresh medium on clinical pregnancy outcomes. A total of 318 fresh IVF/intracytoplasmic sperm injection (ICSI) cycles were randomly allocated into two subgroups based on their transfer media (using a self-spent culture medium or new pre-equilibrated culture media), and the clinical outcomes were compared between groups. The implantation rates, clinical pregnancy rates and live birth rates for transfer using self-spent medium instead of new pre-equilibrated culture medium were slightly improved without statistical significance. Interestingly, however, biochemical pregnancy rate was found to be significantly decreased after transfer using self-spent medium for Day 3 embryos compared with new pre-equilibrated culture media. In short, embryo transfer with self-spent culture medium has shown some advantages, and large sample size studies are still needed to confirm these observations.
ART: assisted reproductive technologies; ICSI: intracytoplasmic sperm injection; IVF: in vitro fertilization; ET: embryo transfer.
胚胎移植是受精（IVF）的最后也是关键的一步，一直以来都受到了广泛关注，并值得不断优化。在本研究中，为了探索自分泌因子在胚胎自我培养中的作用，我们前瞻性地比较了胚胎移植与自我培养和新鲜培养基在临床妊娠结局中的作用。将 318 个新鲜的体外受精/胞浆内单精子注射（ICSI）周期随机分为两组，根据转移介质（使用自我培养介质或新预平衡培养介质），比较组间的临床结局。使用自我培养介质代替新预平衡培养介质进行转移的胚胎着床率、临床妊娠率和活产率略有提高，但无统计学意义。有趣的是，然而，与新预平衡培养介质相比，使用自我培养介质转移第 3 天胚胎的生化妊娠率明显下降。总之，胚胎移植使用自我培养介质具有一定的优势，仍需要大样本量的研究来证实这些观察结果。
ART：辅助生殖技术；ICSI：胞浆内单精子注射；IVF：体外受精；ET：胚胎移植。
