Unité de recherche « Valorisation des biomolécules actives », Institut Supérieur de Biologie Appliquée de Médenine, Route El Jorf - Km 22.5 - 4119 Medenine, Université de Gabès, Gabès, Tunisia.
Laboratoire de Génie Chimique, UMR 5503, Université de Toulouse, CNRS, INPT, UPS, Toulouse, France.
J Food Sci. 2020 Jun;85(6):1856-1871. doi: 10.1111/1750-3841.15157. Epub 2020 May 31.
Brassica tournefortii is an annual herbaceous plant, native to the North Africa and Middle East. It is considered as an excellent medicinal plant due to its richness by antioxidant like isothiocyanates and polyphenols. The present study is the first phytochemical investigation on Brassica tournefortii organs (leaves, stems, and roots) in terms of nutraceutical, chemical composition, and bioactivity. Brassica tournefortii leaves exhibited the highest values of nutraceutical contents. Interestingly, gas chromatograph-y-mass spectrometry (GC-MS) analysis enabled to identify three new isothiocyanates: iberverin nitrile and iberin detected only in roots, and iberin nitrile detected in all organs. HPLC chromatograms displayed different profiles depending on organic solvent and extracted organ. Icariin and 5,7-dihydroxy 4-propylcoumarin showed the highest concentrations with 2.3 and 1.3 mg/g of dr among other molecules identified by high performance liquid chromatography (HPLC). Some phenolic compounds were identified in more than one organ extracts such as phenoxodiol and 4-hydroxy-3-propylbenzoic acid methyl ester. Brassica tournefortii extracts showed a moderate total phenolic contents and anti-15-LOX activity, while they exhibited a good anti-α-glucosidase activity ranging from 40% to 60%. Furthermore, leaves-MeOH and root-dichloromethane (DCM) extracts induced the highest cytotoxicity against MCF-7 cell lines, while roots-cyclohexane (CYHA) extract highlighted the highest inhibition activity against, both, HCT-116 and OVCAR cell lines.
Brassica tournefortii 是一种一年生草本植物，原产于北非和中东。由于其富含抗氧化剂如异硫氰酸盐和多酚，因此被认为是一种极好的药用植物。本研究首次对 Brassica tournefortii 的器官（叶、茎和根）进行了植物化学研究，包括营养成分、化学成分和生物活性。 Brassica tournefortii 的叶子表现出最高的营养成分含量。有趣的是，气相色谱-质谱联用 (GC-MS) 分析能够鉴定出三种新的异硫氰酸盐：仅在根部检测到的iberverin nitrile 和 iberin，以及在所有器官中检测到的 iberin nitrile。高效液相色谱 (HPLC) 分析显示，根据有机溶剂和提取器官的不同，HPLC 图谱显示出不同的图谱。icariin 和 5,7-二羟基 4-丙基香豆素的浓度最高，分别为 2.3 和 1.3mg/g 的干重，其他鉴定出的分子也具有较高的浓度。一些酚类化合物在多个器官提取物中被鉴定出来，如 phenoxodiol 和 4-羟基-3-丙基苯甲酸甲酯。Brassica tournefortii 提取物的总酚含量和抗 15-LOX 活性中等，而抗α-葡萄糖苷酶活性较好，范围为 40%至 60%。此外，叶-MeOH 和根-二氯甲烷 (DCM) 提取物对 MCF-7 细胞系表现出最高的细胞毒性，而根-环己烷 (CYHA) 提取物对 HCT-116 和 OVCAR 细胞系的抑制活性最高。
