[有氧运动对高脂饮食喂养小鼠肝脏中CLK2蛋白表达的影响]
[Effect of aerobic exercise on the expression of CLK2 protein in liver of mice fed with high fat diet].
作者信息
Lu Man-Man, Chen Jia-Yao, Niu Ming-Yu, Wu Wei-Dong
机构信息
College of Physical Education, Guangxi University, Nanning 530001.
Guigang People's Hospital, Guigang 537100.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2020 Jan 28;36(1):23-26. doi: 10.12047/j.cjap.5915.2020.005.
OBJECTIVE
To investigate the effects of aerobic exercise on Cdc2-like kinase (CLK2) protein expression and the fat content in liver of mice fed with high fat diet.
METHODS
C57BL/6 mice were distributed in normal diet, high fat diet (fed with highfat diet during 16 weeks) and trained high fat diet group (fed with high-fat diet during 16 weeks and exercised during 8 weeks),10 mice in each group. The expression of CLK2 protein in liver of each group was detected by Western blot. The fat content of liver in each group was detected by oil red O staining, and the relative genes of fat metabolism in each group were evaluated by real-time quantitative PCR.
RESULTS
The mice fed with high fat diet showed insulin resistance, the hepatic CLK2 content and fat content were increased compared to the normal diet group. Otherwise, the chronic physical exercise improved insulin resistance state, prevented the increasing of CLK2 in the liver and attenuated hepatic fat accumulation.
CONCLUSION
Aerobic exercise could reduce the expression of CLK2 protein in the liver of mice fed with high fat diet.
目的
探讨有氧运动对高脂饮食喂养小鼠肝脏中Cdc2样激酶（CLK2）蛋白表达及脂肪含量的影响。
方法
将C57BL/6小鼠分为正常饮食组、高脂饮食组（高脂饮食16周）和高脂饮食训练组（高脂饮食16周并运动8周），每组10只。采用蛋白质免疫印迹法检测各组小鼠肝脏中CLK2蛋白的表达。用油红O染色法检测各组小鼠肝脏的脂肪含量，并用实时定量PCR法评估各组脂肪代谢相关基因。
结果
高脂饮食喂养的小鼠出现胰岛素抵抗，与正常饮食组相比，其肝脏中CLK2含量和脂肪含量增加。此外，长期体育锻炼改善了胰岛素抵抗状态，阻止了肝脏中CLK2的增加，并减轻了肝脏脂肪堆积。
结论
有氧运动可降低高脂饮食喂养小鼠肝脏中CLK2蛋白的表达。
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