Wang Su-Xing, Wei Jin-Gang, Shao Wei-Hua, Lyu Cai-Xia, Yuan Xiao-Ye
The Second Department of Geriatrics, Hebei General Hospital, Shijiazhuang 050051.
Department of General Surgery, the Fifth Hospital of Shijiazhuang City, Shijiazhuang 050000, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2020 Jan 28;36(1):85-89. doi: 10.12047/j.cjap.5892.2020.019.
To observe the effects of resveratrol on body composition in adult catch-up growth rats and to explore the possible mechanism.
Eight-week-old male SD rats were randomly divided into 6 groups: normal controls for 4 weeks (NC4) group, caloric restriction for 4 weeks (R4) group, calorie restriction meanwhile resveratrol treatment for 4 weeks (R4E) group, normal controls for 12 weeks (NC12) group, catch-up growth (CUG) group and catch-up growth meanwhile resveratrol treatment for 8 weeks (CUGE) group. At the end of the four-week and twelve-week experimental period, the body weight, muscle and fat content of trunk and whole body, the ratio of trunk to whole body fat were detected, and at the end of twelve-week experimental period, the expression of SIRT1 in skeletal muscle and epididymal adipose tissue, and the expression of PPARγ in epididymal adipose tissue were detected.
Compared with NC12 group, the fat content of trunk and whole body and trunk to whole body fat ratio in CUG group were increased significantly, along with the expression of PPARγ in epididymal adipose tissue was increased significantly (P＜0.05), while the muscle content of trunk and whole body, the expression of SIRT1 in skeletal muscle and epididymal adipose tissue in CUG group were decreased significantly compared with NC12 group (P＜0.05 or P＜0.01); compared with CUG group, oral administration of resveratrol distinctly reduced the body fat content and trunk to whole body fat ratio in the CUGE groups, and the expression of PPARγ in epididymal adipose tissue of CUGE group was also significantly decreased (P＜0.05). Meanwhile, the muscle content and the expression of SIRT1 in skeletal muscle and epididymal adipose tissue in CUGE group were significantly increased compared with the CUG group (P＜0.05).
Resveratrol can decrease body fat content, increase muscle content and improve abdominal fat accumulation in adult catch-up growth rats, and its mechanism may be associated with increasing SIRT1 expression in skeletal muscle and visceral adipose tissue, decreasing PPARγ expression in visceral adipose tissue.
观察白藜芦醇对成年期追赶生长大鼠身体成分的影响，并探讨其可能机制。
将8周龄雄性SD大鼠随机分为6组：正常对照4周（NC4）组、热量限制4周（R4）组、热量限制同时白藜芦醇处理4周（R4E）组、正常对照12周（NC12）组、追赶生长（CUG）组和追赶生长同时白藜芦醇处理8周（CUGE）组。在4周和12周实验期结束时，检测体重、躯干及全身肌肉和脂肪含量、躯干与全身脂肪比例，在12周实验期结束时，检测骨骼肌和附睾脂肪组织中SIRT1的表达以及附睾脂肪组织中PPARγ的表达。
与NC12组相比，CUG组躯干及全身脂肪含量、躯干与全身脂肪比例显著增加，附睾脂肪组织中PPARγ表达显著升高（P＜0.05），而CUG组躯干及全身肌肉含量、骨骼肌和附睾脂肪组织中SIRT1表达与NC12组相比显著降低（P＜0.05或P＜0.01）；与CUG组相比，口服白藜芦醇明显降低了CUGE组体脂含量和躯干与全身脂肪比例，CUGE组附睾脂肪组织中PPARγ表达也显著降低（P＜0.05）。同时，CUGE组肌肉含量以及骨骼肌和附睾脂肪组织中SIRT1表达与CUG组相比显著增加（P＜0.05）。
白藜芦醇可降低成年期追赶生长大鼠体脂含量，增加肌肉含量，改善腹部脂肪堆积，其机制可能与增加骨骼肌和内脏脂肪组织中SIRT1表达、降低内脏脂肪组织中PPARγ表达有关。
