Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Exp Biol Med (Maywood). 2013 Jan;238(1):57-65. doi: 10.1258/ebm.2012.012207.
Catch-up growth in adult (CUGA) is increasingly proposed as an important causative factor for the widespread insulin resistance (IR)-related diseases especially in developing countries/territories. We aimed to investigate the effects of CUGA to insulin sensitivity, lipid profile and stress in rats, as well as the probable relationship among them. Male Sprague-Dawley rats were randomly divided into six groups for two sampling points: caloric restriction group (R4) and normal chow controls for four weeks (NC4); CUGA re-fed with normal chow (RN4), CUGA re-fed with high-fat diet (RH4), normal chow controls (NC8) and high-fat diet controls (HF8) for eight weeks. Visceral fat accumulation (visceral adipose tissue [VAT] percentage), systemic (plasma corticosterone) and local (HSD11B1 mRNA expression in skeletal muscle [SkM] and VAT) stress, whole-body and peripheral insulin sensitivity were determined in this study. After four weeks of caloric restriction, R4 rats showed increases in systemic and local stress, decreases in visceral fat accumulation and no IR (whole-body or peripheral). Yet, after re-feeding, sustained systemic and local stress, remarkable visceral fat accumulation and IR (whole-body and peripheral) were found in RN4 compared with NC8, in RH4 compared with NC8 and HF8. Our findings demonstrated that CUGA rats were characterized by significant IR, visceral fat accumulation and stress. These changes were more severe in CUGA re-fed with high-fat diet. The interaction of sustained caloric restriction-induced stress and re-feeding might be of utmost importance in the etiology of visceral fat accumulation and IR in CUGA.
成人追赶生长（CUGA）作为一个重要的致病因素，被越来越多地提出与胰岛素抵抗（IR）相关疾病有关，尤其在发展中国家/地区。本研究旨在探讨 CUGA 对大鼠胰岛素敏感性、血脂谱和应激的影响，以及它们之间可能的关系。雄性 Sprague-Dawley 大鼠随机分为两组，每组再分为 3 个亚组，共 6 个亚组，在两个时间点进行采样：热量限制组（R4）和正常饮食对照组（NC4），分别喂养 4 周；CUGA 恢复正常饮食组（RN4）、CUGA 恢复高脂饮食组（RH4）、正常饮食对照组（NC8）和高脂饮食对照组（HF8），喂养 8 周。本研究测定了内脏脂肪堆积（内脏脂肪组织 [VAT]百分比）、全身（血浆皮质酮）和局部（骨骼肌 [SkM]和 VAT 中 HSD11B1 mRNA 表达）应激、全身和外周胰岛素敏感性。热量限制 4 周后，R4 大鼠表现出全身和局部应激增加，内脏脂肪堆积减少，无 IR（全身或外周）。然而，重新喂养后，RN4 与 NC8 相比，RH4 与 NC8 和 HF8 相比，大鼠持续存在全身和局部应激，显著的内脏脂肪堆积和 IR（全身和外周）。我们的研究结果表明，CUGA 大鼠表现出显著的 IR、内脏脂肪堆积和应激。在 CUGA 大鼠重新喂养高脂饮食后，这些变化更为严重。持续的热量限制诱导的应激和重新喂养的相互作用可能在 CUGA 中内脏脂肪堆积和 IR 的发病机制中至关重要。
