Kovalenko A G, Grabina T D, Bobyr' A D, Korbelaĭnen E S, Vitovskaia G A
Vopr Virusol. 1988 Nov-Dec;33(6):732-7.
Mannan sulphates (MS) synthesized on the basis of extracellular linear mannan (LM) of Rhodotorula rubra induce resistance of Immune-580 tobacco and thornapple (Datura stramonium) to tobacco mosaic virus (TMV). The resistance is manifested in a decrease in the number and/or size of viral local lesions (LL) in MS-treated (mg/ml) leaf halves. The reduction in the LL size does not seem to be due to the direct inhibition of TMV multiplication by the polysaccharide as the virus accumulation in the tissues of the systemic host (Samsun tobacco) does not decrease when MS is used 15 min after infection. The induced resistance is partially inhibited by actinomycin D and completely by heating (32 degrees C). Unlike MS, neutral LM decreases only the number of LL on the treated parts of the tissue but does not influence their size. The antiviral activity of LM does not change in the presence of actinomycin D. The possible mechanisms of the LM and MS protective effect in plants are discussed.
基于红酵母胞外线性甘露聚糖（LM）合成的甘露聚糖硫酸盐（MS）可诱导免疫580烟草和曼陀罗对烟草花叶病毒（TMV）产生抗性。这种抗性表现为经MS处理（毫克/毫升）的半叶叶片中病毒局部病斑（LL）的数量和/或大小减少。病斑大小的减小似乎并非由于多糖对TMV增殖的直接抑制，因为在感染后15分钟使用MS时，系统性寄主（三生烟草）组织中的病毒积累并未减少。诱导抗性部分受到放线菌素D的抑制，加热（32摄氏度）则可完全抑制。与MS不同，中性LM仅减少组织处理部位的病斑数量，但不影响其大小。在放线菌素D存在的情况下，LM的抗病毒活性不变。文中讨论了LM和MS在植物中的保护作用的可能机制。
