Kovalenko A G, Shcherbatenko I S, Kogan E M, Platonova G A, Tikhomirova-Sidorova N S
Vopr Virusol. 1980 May-Jun(3):364-8.
Inoculation of double-stranded polyribonucleotide poly(G) . poly(C) complex in a concentration of 50-200 micrograms/mg into tobacco and thornapple leaves was found to produce resistance of the plants to subsequent infection with tobacco mosaic virus (TMV) manifested in decreased number and size of local virus lesions. The induced resistance may spread over the plant and be found in the upper untreated leaves. The level of systemic resistance, however, is much lower than that of the resistance demonstrated in the injected leaves. Actinomycin D (5 micrograms/ml) had no significant effect on the number but stimulated the growth of lesions developing in leaves injected with poly(G) . poly(C) as well as increased their number in the upper leaves of the same plants proximal to the treated ones. Development of tobacco resistance to TMV was accompanied by changes in the activity of terminal oxidases, particularly peroxidase. Possible mechanisms of formation of induced resistance are discussed.
将浓度为50-200微克/毫克的双链多聚核糖核苷酸聚（G）.聚（C）复合物接种到烟草和曼陀罗叶片中，发现可使植株对随后感染烟草花叶病毒（TMV）产生抗性，表现为局部病毒病斑的数量和大小减少。诱导抗性可在植株内扩散，并在未处理的上部叶片中发现。然而，系统抗性水平远低于注射叶片中表现出的抗性水平。放线菌素D（5微克/毫升）对病斑数量没有显著影响，但刺激了注射聚（G）.聚（C）的叶片中病斑的生长，并增加了同一植株靠近处理叶片的上部叶片中的病斑数量。烟草对TMV抗性的产生伴随着末端氧化酶，特别是过氧化物酶活性的变化。文中讨论了诱导抗性形成的可能机制。
