Qu Yue, Li Yali, Cameron David R, Easton Christopher D, Zhu Xuebo, Zhu Minli, Salwiczek Mario, Muir Benjamin W, Thissen Helmut, Daley Andrew, Forsythe John S, Peleg Anton Y, Lithgow Trevor
The Neonatal Intensive Care Unit, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
Infection and Immunity Theme, Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.
Front Microbiol. 2020 May 13;11:920. doi: 10.3389/fmicb.2020.00920. eCollection 2020.
is an opportunistic pathogen often implicated in bloodstream infections in the neonatal intensive care unit (NICU). This is assisted by its ability to form biofilms on indwelling central venous catheters (CVC), which are highly resistant to antibiotics and the immune system. We sought to understand the fundamentals of biofilm formation by in the NICU, using seventeen clinical isolates including the endemic NRCS-A clone and assessing nine commercial and two modified polystyrene surfaces. clinical isolates from the NICU initiated biofilm formation only in response to hyperosmotic conditions, followed by a developmental progression driven by expression to establish mature biofilms, with polysaccharide being their major extracellular polymer substance (EPS) matrix component. Physicochemical features of the biomaterial surface, and in particular the level of the element oxygen present on the surface, significantly influenced biofilm development of . A lack of highly oxidized carbon species on the surface prevented the immobilization of EPS and the formation of mature biofilms. This information provides guidance in regard to the preparation of hyperosmolar total parenteral nutrition and the engineering of CVC surfaces that can minimize the risk of catheter-related bloodstream infections caused by in the NICU.
是一种机会致病菌，常与新生儿重症监护病房（NICU）的血流感染有关。它能够在留置中心静脉导管（CVC）上形成生物膜，这有助于其引发感染，因为生物膜对抗生素和免疫系统具有高度抗性。我们试图通过使用包括地方性NRCS - A克隆在内的17株临床分离株，并评估9种商用和2种改良聚苯乙烯表面，来了解NICU中生物膜形成的基本原理。来自NICU的临床分离株仅在高渗条件下才开始形成生物膜，随后在表达的驱动下经历发育进程以建立成熟生物膜，多糖是其主要的细胞外聚合物物质（EPS）基质成分。生物材料表面的物理化学特征，特别是表面存在的氧元素水平，显著影响了生物膜的发育。表面缺乏高度氧化的碳物种会阻止生物膜EPS的固定和成熟生物膜的形成。这些信息为高渗全胃肠外营养的制备以及CVC表面工程提供了指导，从而可以最大限度地降低NICU中由生物膜引发的导管相关血流感染的风险。
