耐环丙沙星人型支原体临床分离株的基因组结构
The Genome Structure of Ciprofloxacin-Resistant Mycoplasma Hominis Clinical Isolates.
作者信息
Kolesnikova E A, Brusnigina N F, Makhova M A, Alekseeva A E
机构信息
Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology, Federal Service for Surveillance on Customers Rights Protection and Human Wellbeing, Nizhniy Novgorod, 603950 Russia.
出版信息
Acta Naturae. 2020 Jan-Mar;12(1):56-62. doi: 10.32607/actanaturae.10941.
The genome structure of three ciprofloxacin-resistant clinical isolates was studied using next-generation sequencing on the Illumina platform. The protein sequences of the studied strains were found to have a high degree of homology. (M45, M57, MH1866) was shown to have limited biosynthetic capabilities, associated with the predominance of the genes encoding the proteins involved in catabolic processes. Multiple single-nucleotide substitutions causing intraspecific polymorphism of were found. The genes encoding the efflux systems - ABC transporters (the ATP-binding cassette superfamily) and proteins of the MATE (multidrug and toxic compound extrusion) family - were identified. The molecular mechanism of ciprofloxacin resistance of the M45 and M57 isolates was found to be associated with the Ser83Leu substitution in DNA gyrase subunit A. In the MH1866 isolate it was related to the Lys144Arg substitution in topoisomerase IV subunit A.
使用Illumina平台上的下一代测序技术研究了三株耐环丙沙星临床分离株的基因组结构。研究发现这些菌株的蛋白质序列具有高度同源性。（M45、M57、MH1866）显示出有限的生物合成能力，这与编码参与分解代谢过程的蛋白质的基因占优势有关。发现了多个导致种内多态性的单核苷酸替换。鉴定出了编码外排系统的基因——ABC转运蛋白（ATP结合盒超家族）和MATE（多药和有毒化合物外排）家族的蛋白质。发现M45和M57分离株对环丙沙星耐药的分子机制与DNA促旋酶亚基A中的Ser83Leu替换有关。在MH1866分离株中，它与拓扑异构酶IV亚基A中的Lys144Arg替换有关。
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